Comparative Study of Reprogramming Efficiency and Regulatory Mechanisms of Placental- and Fibroblast-Derived Induced Pluripotent Stem Cells (iPSCs) in Mules.

Fangyuan Liu, Jia Zhang, Lingyu Kong, Rihan Wu, Qiqi Jiang, Ying Lu, Xihe Li

Abstract read

In one paragraph

Article in Current issues in molecular biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

7 authors.

Fangyuan LiuState Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China.

Jia ZhangState Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China.

Lingyu KongCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.

Rihan WuState Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China.

Qiqi JiangState Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China.

Ying LuCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.ORCID 0000-0002-2509-9209

Xihe LiState Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China.ORCID 0000-0002-7001-7997

Funding

Inner Mongolia Autonomous Region "Jiebangguashuai" project of China No. 2022JBGS0021

6 · The paper itself

Abstract

As an interspecies hybrid inheriting genetic material from horse and donkey lineages, mules provide a unique model for studying allele-specific regulatory dynamics. Here, we isolated adult fibroblasts (AFs) and placental fibroblasts (PFs) from mule tissues and reprogrammed them into induced pluripotent stem cells (iPSCs). Intriguingly, placental fibroblast-derived iPSCs (mpiPSCs) exhibited reduced reprogramming efficiency compared to adult fibroblast-derived iPSCs (maiPSCs). Through allele-specific expression (ASE) analysis, we systematically dissected transcriptional biases in parental cell types and their reprogrammed counterparts, revealing conserved preferential expression of asinine alleles in core pluripotency regulators (e.g.,

Indexed as

ASEinterspecies hybridiPSCsPI3K-AKTpluripotencyreprogramming

Identifiers

PMID40864824

DOI10.3390/cimb47080671

PMCPMC12384536

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.