ReviewAnalytical sciences : the international journal of the Japan Society for Analytical Chemistry2025
Review of methods for the determination of cellular cholesterol content: principles, advantages, limitations, applications, and perspectives.
Review in Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- High-level fed-batch production of recombinant type II cholesterol oxidase fromBiotechnology reports (Amsterdam, Netherlands) · 2026Article
- Sequential Injection Analysis of Cholesterol Using an Oxidation-Reduction Electrode Detector.Sensors (Basel, Switzerland) · 2025Article
Corrections and comments
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Authors and funding
3 authors.
Funding
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Abstract
Imbalances in cellular cholesterol homeostasis are associated with various diseases, and accurate determination of cholesterol levels and distribution is essential for a thorough understanding of cellular physiopathology. In this review, we comprehensively analyzed various techniques for cellular cholesterol determination. They include indirect methods based on SREBP2 activity monitoring, gas chromatography-liquid chromatography coupling, enzyme analysis, improved Abell-Kendall method, cholesterol-specific probes such as filipin III and cholesterol-dependent cytolysin, and cholesterol analogs such as dehydroergosterol, BODIPY-cholesterol, label-free as well as labeling Raman assays and mass spectrometry imaging techniques such as matrix-assisted laser desorption/ionization mass spectrometry imaging, desorption electrospray ionization mass spectrometry imaging, and nanoscale secondary ion mass spectrometry technology. Principles, advantages, and limitations of each technique are discussed in detail, their characteristics in terms of sensitivity, spatial resolution, and temporal resolution are compared in detail. Finally, suggestions for selecting the technique for different experimental objectives are also given. These findings will help researchers choose the most suitable method according to their own needs, provide strong support for cellular cholesterol research, and promote the development of related fields to better elucidate the significance of cellular cholesterol in normal biologic activities and also its intrinsic relationship with diseases.
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Registered trials
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