Development of an Ex Vivo Platform to Model Urethral Healing.

Christopher Foster, Ryan Tran, Khushi Grover, Abdullah Salama, Courtney K Rowe

Abstract read

In one paragraph

Article in Methods and protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Christopher FosterDepartment of Pediatrics, University of Connecticut School of Medicine, Farmington, CT 06032, USA.

Ryan TranCollege of Osteopathic Medicine, University of New England, Biddeford, ME 04005, USA.

Khushi GroverDivision of Pediatric Urology, Connecticut Children's, Hartford, CT 06108, USA.

Abdullah SalamaDepartment of Pediatrics, University of Connecticut School of Medicine, Farmington, CT 06032, USA.

Courtney K RoweDepartment of Pediatrics, University of Connecticut School of Medicine, Farmington, CT 06032, USA.ORCID 0000-0002-3309-4897

Funding

Connecticut Children's Connections NA

6 · The paper itself

Abstract

backgroundUrethral strictures impact millions, causing significant morbidity and millions in healthcare costs. Testing new interventions is limited by the lack of inexpensive urethral healing models. We developed an ex vivo model of early urethral wound healing using explanted rabbit urethral tissue. This was used to test the impact of six growth factors (GFs).

methodsThe rabbit urethra was detubularized by cutting it between the corpora cavernosa, and then it was stitched flat using a custom 3D-printed platform. The tissue was carefully scratched to produce a visible wound, and the specimens were placed in media containing growth factors at 100 ng/mL and 10 ng/mL. Images were taken at 0, 24, 48, 72, and 96 h, and the wound area was measured by blinded reviewers to determine the rate of wound contraction.

resultsSpecimens with IGF at 100 ng/mL showed a statistically significant difference in wound contraction when compared to those with GF-free control medium, showing that IGF-1 supports early urethral epithelization and may improve healing.

conclusionsThe developed protocol provides a simple explant platform that can be used to investigate methods of enhancing early phases of urethral healing or used to investigate other areas of urethral health, including drug delivery, infection, and mechanical properties.

Indexed as

growth factorregenerative medicineurethrawound healing

Identifiers

PMID40863746

DOI10.3390/mps8040096

PMCPMC12388648

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.