Enhanced Tangential Flow Filtration of Precipitated Proteins Using Screened Membrane Cassettes.

Zachary Badinger, Ali Behboudi, Andrew L Zydney

Abstract read

In one paragraph

Article in Membranes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Zachary BadingerDepartment of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802, USA.

Ali BehboudiDepartment of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802, USA.ORCID 0000-0003-4719-6998

Andrew L ZydneyDepartment of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802, USA.ORCID 0000-0003-1865-9156

Funding

U.S. Food and Drug Administration 75F40121C00111

6 · The paper itself

Abstract

backgroundRecent advances in cell culture have led to significant increases in monoclonal antibody (mAb) titers, opening a new window of opportunity for developing a fully continuous downstream purification process based on the selective precipitation of the mAb from harvested cell culture fluid, with the precipitate dewatered and washed using single-pass tangential flow filtration (SPTFF) with microfiltration membranes.

methodsExperiments were performed with precipitates of human serum immunoglobulin G formed using ZnCl

resultsCritical fluxes as high as 250 L/m

conclusionsThe results demonstrate the potential of using screened membrane cassettes for the continuous/intensified processing of precipitated proteins like monoclonal antibodies.

Indexed as

bioprocessingmonoclonal antibodyprotein precipitationscreened cassettetangential flow filtration

Identifiers

PMID40863606

DOI10.3390/membranes15080245

PMCPMC12388320

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.