Evidence map›Paper›PMID 40863325›Full record

ArticleJournal of xenobiotics2025

Development and Validation of LC-MS/MS and IC-HRMS Methods for Highly Polar Pesticide Detection in Honeybees: A Multicenter Study for the Determination of Pesticides in Honeybees to Support Pollinators and Environmental Protection.

Tommaso Pacini, Emanuela Verdini, Serenella Orsini, Katia Russo, Tabita Mauti, Mara Gasparini, Marialuisa Borgia, Barbara Angelone, Teresa D'Amore, Ivan Pecorelli

Abstract read
In one paragraph

Article in Journal of xenobiotics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Tommaso PaciniChemistry Department, Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche "Togo Rosati", Via G. Salvemini, 1, 06126 Perugia, Italy.ORCID 0000-0003-4872-7623
Emanuela VerdiniChemistry Department, Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche "Togo Rosati", Via G. Salvemini, 1, 06126 Perugia, Italy.ORCID 0000-0002-8146-600X
Serenella OrsiniChemistry Department, Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche "Togo Rosati", Via G. Salvemini, 1, 06126 Perugia, Italy.ORCID 0000-0002-3018-4010
Katia RussoDirezione Operativa Chimica, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana "M. Aleandri", Via Appia Nuova 1411, 00178 Rome, Italy.ORCID 0000-0002-8355-6869
Tabita MautiDirezione Operativa Chimica, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana "M. Aleandri", Via Appia Nuova 1411, 00178 Rome, Italy.
Mara GaspariniFood and Feed Chemistry Department, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna "Bruno Ubertini", Via Bianchi, 9, 25124 Brescia, Italy.ORCID 0000-0001-9332-0529
Marialuisa BorgiaFood and Feed Chemistry Department, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna "Bruno Ubertini", Via Bianchi, 9, 25124 Brescia, Italy.
Barbara AngeloneFood and Feed Chemistry Department, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna "Bruno Ubertini", Via Bianchi, 9, 25124 Brescia, Italy.
Teresa D'AmoreLaboratory of Preclinical and Translational Research, IRCCS CROB, Basilicata Oncology Referral Center, Rionero in Vulture, 85028 Naples, Italy.ORCID 0000-0002-1435-4390
Ivan PecorelliChemistry Department, Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche "Togo Rosati", Via G. Salvemini, 1, 06126 Perugia, Italy.ORCID 0000-0003-2889-1071

Funding

Ministry of Health Research Project PRC2021009
6 · The paper itself

Abstract

The widespread use of agrochemicals raises concerns about environmental impacts, particularly on pollinators, such as bees, which serve as bioindicators of contamination. Developing methods to assess contamination risks in bioindicators supports regulatory frameworks, including EU regulations on the maximum residue limits (MRLs) for pesticides in food and the environment. This study presents the development and validation of two complementary analytical methods (LC-MS/MS and IC-HRMS) for highly polar pesticide (HPP) detection and quantification in bee matrices. Both methods were validated according to document SANTE/11312/2021 v2. LC-MS/MS was validated with a limit of quantification (LOQ) of 0.005 mg/kg for all the analytes. Repeatability at 0.005, 0.010, 0.020, and 0.100 mg/kg showed RSD

Indexed as

honeybeesIC–HRMS validationLC–MS/MS validationpolar pesticides

Identifiers

PMID40863325
PMCPMC12387874

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.