ArticleCells2025
New Approaches to Old Techniques in Cell Handling for Microscopy.
Article in Cells, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Appropriate concentrations of reagents, an absence of significant cell clumps and debris, minimization of artifacts and ensuring satisfactory cell preservation directly affect the quality of data generated and cannot be overestimated. Traditionally, cells in suspension are prepared using a cytospin, which uses centrifugal force to concentrate and deposit cells onto a glass slide. Adherent cells are traditionally grown on coverslips located on the bottom of the wells of cell culture plates, or using special chamber slide systems. In our laboratory, we developed and tested simplified homemade approaches for both techniques, allowing users to perform large volume cell functional tests followed by microscopy evaluation without a need for a cytospin, special chamber slide systems or the use of round cover slips. We present methods and illustrative examples involving the cellular uptake of self-delivering oligonucleotides in murine splenocytes and in two adherent human tumor cell lines.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.