Evidence map›Paper›PMID 40862106›Full record

Trial reportFrontiers in endocrinology2025

Effect of high intakes of protein-only and carbohydrate-only on plasma metabolites and hormones, in addition to nitrogen excretion.

Matthieu Clauss, Claire Puissant, Nasser Ezzatkhah Bastani, Lotte L K Nielsen, Bjørn Steen Skålhegg, Per Bendix Jeppesen, Jørgen Jensen

Abstract readRandomized Controlled Trial
In one paragraph

Trial report in Frontiers in endocrinology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Matthieu ClaussDepartment of Physical Performance, Norwegian School of Sport Sciences, Oslo, Norway.
Claire PuissantDepartment of Physical Performance, Norwegian School of Sport Sciences, Oslo, Norway.
Nasser Ezzatkhah BastaniDepartment of Nutrition, Division for Molecular Nutrition, University of Oslo, Oslo, Norway.
Lotte L K NielsenDepartment of Clinical Medicine, Aarhus University Hospital, Aarhus, Denmark.
Bjørn Steen SkålheggDepartment of Nutrition, Division for Molecular Nutrition, University of Oslo, Oslo, Norway.
Per Bendix JeppesenDepartment of Clinical Medicine, Aarhus University Hospital, Aarhus, Denmark.
Jørgen JensenDepartment of Physical Performance, Norwegian School of Sport Sciences, Oslo, Norway.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Hormonal and metabolic responses to high protein intake are not well understood. The aims of this study were to compare the metabolic and hormonal responses to isocaloric intakes of whey protein alone versus carbohydrate alone. Additionally, we measured urinary nitrogen excretion as a marker of protein degradation. Methods: Fourteen young, healthy, moderate-to-well-trained participants (VO Results: After CHO intake, plasma glucose levels increased, and certain plasma amino acid concentrations decreased. Following PRO intake, plasma glucose decreased, and plasma amino acids increased. Insulin concentrations increased following ingestion of both CHO and PRO (time effect, p<0.001), with a greater increase in CHO (drink effect, p<0.001). Plasma GLP-1 and GIP concentrations increased in both conditions (time effect, p<0.001). Plasma GLP-1 increased more in PRO than in CHO (drink effect, p<0.001), whereas plasma GIP increased more in CHO than in PRO (drink effect, p<0.001). Urinary nitrogen excretion over the 24 hours following drink ingestion was significantly higher in PRO (p<0.001), particularly between 2 to 8 hours after intake (p<0.001). Conclusions: CHO increased plasma insulin more than PRO. The PRO induced insulin response was independent of glucose and mediated by the increase in plasma amino acids and GLP-1. Interestingly, the GLP-1 response was larger following PRO and remained elevated after 240 minutes, whereas the GIP response was larger following CHO. Additionally, protein-only ingestion increased urinary nitrogen excretion, mainly between 2 to 8 hours after intake, with elevated excretion persisting up to 24 hours.

Indexed as

Dietary CarbohydratesDietary ProteinsHormonesNitrogenWhey ProteinsAdultAmino AcidsBlood GlucoseCross-Over StudiesDouble-Blind MethodFemaleHumansInsulinMaleYoung AdultAmino AcidsBlood GlucoseDietary CarbohydratesDietary ProteinsHormonesInsulinNitrogenWhey ProteinsGIPGLP-1glucagonincretininsulinprotein metabolismtime courseurinary nitrogen excretion

Identifiers

PMID40862106
PMCPMC12375484

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.