ArticleACS measurement science au2025
Method for Mid-IR Spectroscopy of Extracellular Vesicles at the Subvesicle Level.
Article in ACS measurement science au, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT07402083 (Administration of Extracellular Vesicles Isolated From Donor Human Milk as a Dietary Supplement for the Prevention of Necrotizing Enterocolitis in Preterm Infants), which is not on this map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Administration of Extracellular Vesicles Isolated From Donor Human Milk as a Dietary Supplement for the Prevention of Necrotizing Enterocolitis in Preterm Infants
Who cites it
3 citing papers in PubMed.
- Infrared Near-Field Microscopy as a Platform for Nanoscale Biomedical Spectroscopy and Imaging.ACS applied materials & interfaces · 2026Review
- Metabolomics-guided Mechanisms of Mesenchymal Stromal Cell and Exosome Therapies in Chronic Diseases.Stem cell reviews and reports · 2026Review
- Nanochemical Cell-Surface Evaluation in Photothermal Spectroscopic Imaging of Antimicrobial Interactions in the Model SystemAnalytical chemistry · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extracellular vesicles (EVs) are nanosized particles that are associated with various physiological and pathological functions. They play a key role in intercell communication and are used as transport vehicles for various cell components. In human milk, EVs are believed to be important for the development of acquired immunity. State-of-the-art analysis methods are not able to provide label-free chemical information at the single-vesicle level. We introduce a protocol to profile the structure and composition of individual EVs with the help of atomic force microscopy infrared spectroscopy (AFM-IR), a nanoscale chemical imaging technique. The protocol includes the immobilization of EVs onto a silicon surface functionalized with anti-CD9 antibodies via microcontact printing. AFM-IR measurements of immobilized EVs provide size information and mid-infrared spectra at subvesicle spatial resolution. The received spectra compare favorably to bulk reference spectra. A key part of our protocol is a technique to acquire spectral information about a large number of EVs through hyperspectral imaging combined with image processing to correct for image drift and select individual vesicles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.