Evidence map›Paper›PMID 40861495›Full record

ArticleFrontiers in cellular and infection microbiology2025

Deletion of the E3 ubiquitin ligase LRSAM1 fosters intracellular

Ole Plöhn, Abhishek Kumar Singh, Clara Greger, Hannes Wolfgramm, Madina Baglanova, Kristin Surmann, Uwe Völker, Barbara M Bröker, Karsten Becker, Ulrike Seifert and 1 more

Abstract read
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In one paragraph

Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. E3 ubiquitin ligase SKP2 limits autophagosome formation duringFrontiers in cellular and infection microbiology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Ole PlöhnFriedrich Loeffler - Institute of Medical Microbiology, University Medicine Greifswald, Greifswald, Germany.
Abhishek Kumar SinghFriedrich Loeffler - Institute of Medical Microbiology, University Medicine Greifswald, Greifswald, Germany.
Clara GregerFriedrich Loeffler - Institute of Medical Microbiology, University Medicine Greifswald, Greifswald, Germany.
Hannes WolfgrammDepartment of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany.
Madina BaglanovaFriedrich Loeffler - Institute of Medical Microbiology, University Medicine Greifswald, Greifswald, Germany.
Kristin SurmannDepartment of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany.
Uwe VölkerDepartment of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany.
Barbara M BrökerInstitute of Immunology, University Medicine Greifswald, Greifswald, Germany.
Karsten BeckerFriedrich Loeffler - Institute of Medical Microbiology, University Medicine Greifswald, Greifswald, Germany.
Ulrike SeifertFriedrich Loeffler - Institute of Medical Microbiology, University Medicine Greifswald, Greifswald, Germany.
Clemens CammannFriedrich Loeffler - Institute of Medical Microbiology, University Medicine Greifswald, Greifswald, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Intracellular invasion and persistence of Methods: We studied A549 lung epithelial cells during Results: In LRSAM1-deficient host cells we observed a significant increase in intracellular bacterial load, which was accompanied by an increased host cell death and elevated secretion of the pro-inflammatory cytokine IL-6. Despite induced selective autophagy, LRSAM1 knockout host cells were incapable of lowering and eliminating the pathogen, which seems to be caused by the reduced ubiquitination of the bacterial surface. Conclusion: The results indicate a significant role of LRSAM1 in the clearance of intracellular

Indexed as

Host-Pathogen InteractionsMicrobial ViabilityStaphylococcal InfectionsStaphylococcus aureusUbiquitin-Protein LigasesA549 CellsAutophagyBacterial LoadCRISPR-Cas SystemsCytokinesEpithelial CellsGene DeletionHumansInterleukin-6Signal TransductionUbiquitinationCytokinesInterleukin-6Ubiquitin-Protein LigasesE3 ligaseintracellular bacteriaLRSAM1S. aureusselective autophagyubiquitin

Identifiers

PMID40861495
PMCPMC12375608

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.