Evidence map›Paper›PMID 40861485›Full record

ArticleFrontiers in cellular and infection microbiology2025

Comparison of pathogen detection performance between metagenomic next-generation sequencing and conventional culture in organ preservation fluids and recipient wound drainage fluids.

Jiyuan Li, Wenjia Yuan, Chen Gao, Lei Liu, Lei Song, Wei Cao, Xuejing Zhu, Yachun Han, Ruobing Liang, Gongbin Lan and 7 more

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

17 authors.

Jiyuan Li *Department of Kidney Transplantation, Center of Organ Transplantation, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Wenjia Yuan *Department of Kidney Transplantation, Center of Organ Transplantation, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Chen GaoDepartment of Kidney Transplantation, Center of Organ Transplantation, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Lei LiuDepartment of Kidney Transplantation, Center of Organ Transplantation, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Lei SongDepartment of Kidney Transplantation, Center of Organ Transplantation, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Wei CaoDepartment of Medical Laboratory, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Xuejing ZhuDepartment of Nephrology, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Yachun HanDepartment of Nephrology, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Ruobing LiangDepartment of Scientific Affairs, Hugobiotech Co., Ltd., Beijing, China.
Gongbin LanDepartment of Kidney Transplantation, Center of Organ Transplantation, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Shaojie YuDepartment of Kidney Transplantation, Center of Organ Transplantation, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Yu WangDepartment of Kidney Transplantation, Center of Organ Transplantation, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Liang TanDepartment of Kidney Transplantation, Center of Organ Transplantation, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Helong DaiDepartment of Kidney Transplantation, Center of Organ Transplantation, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Xubiao XieDepartment of Kidney Transplantation, Center of Organ Transplantation, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Longkai PengDepartment of Kidney Transplantation, Center of Organ Transplantation, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Fenghua PengDepartment of Kidney Transplantation, Center of Organ Transplantation, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Prompt identification and management of donor-derived infections post-kidney transplantation are critical. This study aims to assess the effectiveness of metagenomic next-generation sequencing (mNGS) in detecting pathogens within donor organ preservation fluids and recipient wound drainage fluids, with a comparison made against conventional culture methods. Methods: This study involved 141 kidney transplant patients (May 1st, 2020 to Jan 31st, 2024). Donor organ preservation fluids and recipient wound drainage fluids were collected and analyzed by mNGS and conventional culture. Pathogen detection differences between mNGS and culture were evaluated. The antibiotic adjustment and infectious complications of the recipients were recorded. Results: For organ preservation fluids, the positive rate of convention culture were lower than that of mNGS (24.8% (35/141) vs 47.5% (67/141), p<0.05). For recipient wound drainage fluids, the positivity rate of convention culture were lower than that of mNGS (2.1% (3/141) vs 27.0% (38/141), p<0.05). Compared to traditional culture-based methods, mNGS demonstrated a significantly higher positive detection rate for the combination of ESKAPE pathogens and/or fungi (28.4% (40/141) vs 16.3% (23/141) Conclusions: By employing mNGS to analyze drainage fluids and organ preservation fluids, highly pathogenic and atypical pathogenic microorganisms can be rapidly identified with high throughput. While limitations exist in detecting fungi and Gram-positive bacteria, mNGS are need to be jointly applied with conventional culture under current conditions.

Indexed as

BacteriaHigh-Throughput Nucleotide SequencingKidney TransplantationMetagenomicsOrgan Preservation SolutionsAdultAgedBody FluidsDrainageFemaleFungiHumansMaleMiddle AgedTissue DonorsWound InfectionOrgan Preservation Solutionsdonor-derived infectiondrainage fluidkidney transplantationmetagenomic next-generation sequencingmicrobial culturepreservation fluid

Identifiers

PMID40861485
PMCPMC12375685

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.