Evidence map›Paper›PMID 40861270›Full record

ArticleFrontiers in cell and developmental biology2025

MRE11 orchestrates porcine oocyte meiotic progression by modulating the spindle assembly checkpoint.

Dandan Zhang, Zaishan Yang, Yongteng Zhang, Fugui Fang, Hongguo Cao, Yunsheng Li, Zubing Cao, Yanfeng Xue, Mianqun Zhang

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In one paragraph

Article in Frontiers in cell and developmental biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Dandan Zhang *Department of Reproductive Medicine, General Hospital of Wanbei Coal Group, Suzhou, China.
Zaishan Yang *Key Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Yongteng Zhang *Key Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Fugui FangKey Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Hongguo CaoKey Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Yunsheng LiKey Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Zubing CaoKey Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Yanfeng XueKey Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Mianqun ZhangKey Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Mre11 is a multisubunit nuclease involved in DNA repair, and its dysfunction often causes DNA damage sensitivity, genomic instability, telomere shortening, and aberrant meiosis. However, the specific roles of Mre11 in porcine oocyte meiosis remain unclear. Methods: In this study, porcine oocytes were treated with the Mre11-specific inhibitor mirin to investigate the function of Mre11 during meiotic maturation. Meiotic progression, spindle and chromosome structure, spindle migration, cytoplasmic actin polymerization, and DNA damage levels were assessed using immunofluorescence and relevant molecular markers including BubR1 and γH2A.X. Results: Inhibition of Mre11 activity led to failure of first polar body extrusion, with sustained BubR1 presence at kinetochores, indicating activation of the spindle assembly checkpoint (SAC). Mre11-inhibited oocytes showed disrupted spindle and chromosome organization due to decreased microtubule stability. Additionally, spindle migration to the oocyte cortex was impaired, correlating with reduced cytoplasmic actin polymerization. Elevated DNA damage levels were observed in treated oocytes as evidenced by increased γH2A.X staining. Discussion: These findings demonstrate that Mre11 is essential for porcine oocyte meiotic progression by maintaining normal spindle assembly, actin cytoskeleton dynamics, and SAC activity. DNA damage accumulation following Mre11 inhibition likely contributes to meiotic failure, highlighting its critical role in ensuring oocyte quality.

Indexed as

actinMRE11oocytes developmentSACspindle

Identifiers

PMID40861270
PMCPMC12370773

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