A mathematical model of cell-free transcription-translation with plasmid crosstalk.

Yue Han, Alexandra T Patterson, Fernanda Piorino, Mark P Styczynski

Abstract read

In one paragraph

Article in Synthetic biology (Oxford, England), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Harnessing Plasmid Crosstalk in Transcription Factor Mediated Cell-Free Biosensors.bioRxiv : the preprint server for biology · 2025
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Yue HanSchool of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, United States.ORCID https://orcid.org/0000-0001-7372-6724

Alexandra T PattersonSchool of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, United States.

Fernanda PiorinoSchool of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, United States.

Mark P StyczynskiSchool of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, United States.ORCID https://orcid.org/0000-0002-1479-6658

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell-free expression (CFE) systems are emerging as a powerful tool in synthetic biology, with diverse applications from prototyping genetic circuits to serving as a platform for point-of-care biosensors. When multiple genes need to be expressed in the same CFE reaction, their DNA templates (often added as plasmids) are generally assumed to behave independently of each other, with neither affecting the other's expression. However, recent work in

Indexed as

Cell-free systemsgene expressionmathematical modellingsynthetic biologysystems biology

Identifiers

PMID40859943

DOI10.1093/synbio/ysaf011

PMCPMC12371409

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.