Evidence map›Paper›PMID 40859084›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Assessment of Angiogenic Properties of Malignant Pleural Effusion (MPE) Fluid.

Constantina Santorinaiou, Margarita Lamprou, Eleni Mourkogianni, Andriana Plevriti, Epameinondas Lyros, Constantinos M Mikelis, Antonia Marazioti

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Constantina SantorinaiouBasic Sciences Laboratory, Department of Physiotherapy, School of Health Sciences, University of the Peloponnese, Tripoli, Greece.
Margarita LamprouLaboratory of Molecular Pharmacology, Department of Pharmacy, School of Health Sciences, University of Patras, Patras, Greece.
Eleni MourkogianniLaboratory of Molecular Pharmacology, Department of Pharmacy, School of Health Sciences, University of Patras, Patras, Greece.
Andriana PlevritiLaboratory of Molecular Pharmacology, Department of Pharmacy, School of Health Sciences, University of Patras, Patras, Greece.
Epameinondas LyrosBasic Sciences Laboratory, Department of Physiotherapy, School of Health Sciences, University of the Peloponnese, Tripoli, Greece.
Constantinos M MikelisLaboratory of Molecular Pharmacology, Department of Pharmacy, School of Health Sciences, University of Patras, Patras, Greece.
Antonia MaraziotiBasic Sciences Laboratory, Department of Physiotherapy, School of Health Sciences, University of the Peloponnese, Tripoli, Greece. a.marazioti@uop.gr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Patients with various malignancies can develop malignant pleural effusion (MPE). MPE is the abnormal accumulation of fluid in the pleural cavity. This fluid usually contains immune cells, tumor cells, and high levels of plasma proteins. This condition is associated with impaired pleural fluid drainage and is considered an immune and angiogenic manifestation of tumors metastasized in the pleura from other thoracic and non-thoracic areas. Tumor-induced pleural vascular hyperpermeability and neoangiogenesis are crucial to pleural fluid accumulation and could be therapeutically targeted to minimize MPE development. Here, we present two methodologies required for assessing the angiogenic properties of MPE fluid-Evans' blue pulse-and-chase for evaluating increased vascular permeability and the chorioallantoic membrane (CAM) assay to assess tumor-triggered angiogenesis. We describe the steps for experimental MPE formation in mice, including MPE fluid collection, evaluation of MPE-induced vascular permeability, and assessment of MPE-induced angiogenesis.

Indexed as

Neovascularization, PathologicPleural Effusion, MalignantAnimalsCapillary PermeabilityChorioallantoic MembraneDisease Models, AnimalEvans BlueHumansMiceEvans BlueAngiogenesisCAM assayCancerMalignant pleural effusionMouse modelPleural fluidPleural metastasisVascular permeability

Identifiers

PMID40859084

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.