Evidence map›Paper›PMID 40859083›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Label-Free Imaging with Automated Tracking and Cell-by-Cell Analysis of Migration, Morphology, and Differentiation of Endothelial Cells.

Jessica Rickman, Meetal Jotangia, James Russell, Martin Humphry

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Jessica RickmanPhase Focus Limited, Electric Works, Sheffield Digital Campus, Sheffield, UK. Jessica.rickman@phasefocus.com.
Meetal JotangiaPhase Focus Limited, Electric Works, Sheffield Digital Campus, Sheffield, UK.
James RussellPhase Focus Limited, Electric Works, Sheffield Digital Campus, Sheffield, UK.
Martin HumphryPhase Focus Limited, Electric Works, Sheffield Digital Campus, Sheffield, UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Endothelial cells line the interior lumen of blood and lymphatic vessels. Tube formation assays recreate the formation of these vessels in vitro. This process is stimulated by the application of vascular endothelial growth factor (VEGF), which binds to the receptor VEGFR2. This protocol outlines the setup and time-lapse imaging of a tube formation assay using Livecyte, a live-cell, label-free, quantitative phase imaging (QPI) microscope. Subsequent software analysis workflows are then described to quantify the cells' mass, motility, morphology, and network formation. Using a skeletonization process, the Livecyte software can quantify the number of nodes, segments, branches, junctions, mean segment length, and total network length over time, allowing researchers to determine the dynamic changes in network formation throughout the assay.

Indexed as

Cell DifferentiationCell MovementCell TrackingEndothelial CellsTime-Lapse ImagingHumansImage Processing, Computer-AssistedNeovascularization, PhysiologicSoftwareVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2Vascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2AngiogenesisCell trackingLabel-freeLive cell imagingMotilitySingle cellSkeletonization

Identifiers

PMID40859083

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.