A New Method to Visualize Retinal Deep Vascular Layers Using Whole-Mount Tissue Preparations.

Julia Migliorati, Georgia Zarkada

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Julia MiglioratiDepartment of Physiology & Neurobiology, University of Connecticut, Storrs, CT, USA.

Georgia ZarkadaDepartment of Physiology & Neurobiology, University of Connecticut, Storrs, CT, USA. Georgia.zarkada@uconn.edu.

Funding

Targeting TGFB signaling to treat ocular neovascular diseaseR00EY029375 · NEI · UNIVERSITY OF CONNECTICUT STORRS · PI ZARKADA, GEORGIA · 2023 to 2025

$772k

NEI NIH HHS R00 EY029375

6 · The paper itself

Abstract

The mouse retina is a widely used model to study sprouting angiogenesis. Most blood vessel staining protocols focus on the superficial vascular layer. Here, we describe a method to obtain thick sections of retina whole-mounts that allow for detailed visualization of diving vascular sprouts, and deep and intermediate-layer angiogenesis. This protocol does not require extra tissue processing and can be coupled with any previously optimized protocol for retina whole-mount staining.

Indexed as

RetinaRetinal VesselsAnimalsMiceNeovascularization, PhysiologicStaining and LabelingAngiogenic sproutingDeep retinal plexusNeuroretina vascularizationRetina sectioningRetina stainingRetina whole-mount protocol

Identifiers

PMID40859079

DOI10.3791/2563

PMCPMC12683669

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.