ArticleMethods in molecular biology (Clifton, N.J.)2025
Unveiling Central Nervous System Pericytes: Immunofluorescence Protocol and Confocal Microscopy.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- Neu5Gc-associated Serum Immunoglobulin G Glycosylation as a Diagnostic Biomarker for Hepatocellular Carcinoma.Journal of clinical and translational hepatology · 2026Article
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4 authors.
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Abstract
Pericytes are enigmatic cells that contribute significantly to central nervous system (CNS) homeostasis, blood-brain barrier (BBB) regulation, angiogenesis, and maintenance of neurovascular integrity. These versatile cells are associated with blood vessels, embedded within the vascular basal lamina (VBL), and closest to endothelial cells (ECs). Their multifaceted roles in CNS physiology, development, and pathology have been implicated in various neurological disorders, such as stroke, Alzheimer's disease, and multiple sclerosis. Immunohistochemistry (IHC) coupled with confocal microscopy imaging has emerged as a powerful tool for elucidating spatial distribution, molecular phenotype, and functional properties of CNS pericytes. Herein, we describe a detailed protocol using alternatively anti-NG2 or anti-PDGFR antibodies, to identify pericytes on high-resolution immunofluorescence, allowing us to study the three-dimensional network of anatomical interactions between these cells and the surrounding CNS parenchyma.
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