Evidence map›Paper›PMID 40859076›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Studying the Vascular Tone of Mouse Aortic Rings in a Standard Wire Myograph.

Sosanna Kritikou, Cristina Perez-Ternero, Evangelia Papadimitriou, Stavros Topouzis

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Sosanna KritikouLaboratory of Molecular Pharmacology, Department of Pharmacy, University of Patras, Patras, Greece.
Cristina Perez-TerneroCentre for Cardiovascular Medicine and Devices, William Harvey Research Institute, Barts & The London School of Medicine & Dentistry, Queen Mary University of London, London, UK.
Evangelia PapadimitriouLaboratory of Molecular Pharmacology, Department of Pharmacy, University of Patras, Patras, Greece.
Stavros TopouzisLaboratory of Molecular Pharmacology, Department of Pharmacy, University of Patras, Patras, Greece. stto@upatras.gr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Wire myography is a widely used method to study the influence of vasoactive agents on the vascular tone and the contractile properties of blood vessels ex vivo. The literature has several papers with regard to the theoretical background and basic guidelines of myography. Here, we propose a highly detailed practical guide for studying the functional responses of mouse aorta to pharmacological stimuli that modify vascular tone. We have included photographs taken during the dissection and mounting of aortic rings, screenshots from representative LabChart diagrams, as well as a table with "classical" reagents used to probe vascular reactivity, with information on their mechanism of action and the range of concentrations commonly used.

Indexed as

AortaMyographyAnimalsMiceMuscle, Smooth, VascularVasoconstrictionVasoconstrictor AgentsVasoconstrictor AgentsAortaBlood vesselEndotheliumIsolated aortic ringsMouseSmooth muscleVascular reactivityVasocontractionVasorelaxationWire myograph

Identifiers

PMID40859076

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.