Evidence map›Paper›PMID 40859074›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Human iPSC-Derived Astrocyte-Endothelial Cell and Neuron-Endothelial Cell Co-culture Models of the Blood-Brain Barrier to Study the Impact of Ischemic Stroke in Vitro.

Nausheen Syeara, Yash Mehta, Vardan T Karamyan, Abraham Al-Ahmad

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Nausheen SyearaTexas Tech University Health Sciences Center, Jerry H. Hodge School of Pharmacy, Amarillo, TX, USA.
Yash MehtaTexas Tech University Health Sciences Center, Jerry H. Hodge School of Pharmacy, Amarillo, TX, USA.
Vardan T KaramyanTexas Tech University Health Sciences Center, Jerry H. Hodge School of Pharmacy, Amarillo, TX, USA.
Abraham Al-AhmadTexas Tech University Health Sciences Center, Jerry H. Hodge School of Pharmacy, Amarillo, TX, USA. abraham.al-ahmad@ttuhsc.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The role of the blood-brain barrier (BBB) in pathogenic mechanisms of neurodegenerative disorders, including stroke, is well recognized. In vitro BBB models offer opportunities to recapitulate aspects of the disease using different cell types, extracellular matrices, and soluble factors to study the biology of the disease and the role of the BBB. This chapter describes the rigorous protocols and our schemes to successfully establish human-induced pluripotent stem cell-derived astrocyte-endothelial cell and neuron-endothelial cell co-culture models to study the impact of ischemia-reoxygenation for in vitro stroke studies.

Indexed as

AstrocytesBlood-Brain BarrierEndothelial CellsInduced Pluripotent Stem CellsIschemic StrokeNeuronsCell DifferentiationCells, CulturedCoculture TechniquesHumansAstrocytesBloodBrain barrierEndothelial cellsInduced pluripotent stem cellsNeurons

Identifiers

PMID40859074

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.