ArticleCell death discovery2025
Non-uniform impact of extracellular osmotic variations at subcellular level.
Article in Cell death discovery, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Abstract
Osmotic perturbations, towards understanding basic cellular architectures and to alter cellular mechanics for various purposes, are widely utilized in cell biology. While osmotic perturbations are known to alter whole-cell morphology, their subcellular-level impacts remain poorly characterized. Here, we employ a novel quantitative imaging workflow to demonstrate that extracellular osmolarity induces organelle-specific redistribution patterns in adherent RAW264.7 macrophages, independent of whole-cell morphological changes. At the whole-cell level, we report a decrease in cellular pleomorphism (pixel-intensity-distribution-based heterogeneity) under non-isotonic conditions, with cell membrane and lysosomal pleomorphism decreasing as osmolarity decreases. Remarkably, osmolarity-induced variations observed at whole-cell level are translated to actin and tubulin variations only while nucleus, mitochondria, and endoplasmic reticulum are independent of the whole cell morphology alterations. However, there appears to be 'counterbalancing' of lateral polarity in the distributions of nucleus and endoplasmic reticulum in hypo-osmotic conditions. This work promises to be a key contribution towards understanding cellular architectures.
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