ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025
Real-Time Detection of Reduced Nitroreductase with a Reversible Fluorescent Probe.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed.
- NADPH-depletion micelles sensitizing apoptotic and ferroptotic cancer therapy under hypoxia.Materials today. Bio · 2026Article
- A genetic reporter for visualizing nitroreductase activity using magnetic resonance imaging.Chemistry methods : new approaches to solving problems in chemistry · 2026Article
- Real-Time Detection of Reduced Nitroreductase with a Reversible Fluorescent Probe.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
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Authors and funding
9 authors.
Funding
Abstract
Nitroreductase (NTR), a class of flavin-dependent redox enzymes, is a key biomarker for hypoxic tumors. Numerous fluorescent NTR probes have been developed to study hypoxia and associated tumors; however, they are reaction-based and provide only static information on the accumulated enzyme activity at a given time. Reversible binding probes are needed to monitor the enzyme level in real time. Here, the first reversible binding probe is presented that selectively detects the active, reduced form of NTR (red-NTR) with a fluorescence turn-on response. This probe, a benzocoumarin dye functionalized with a (nitrobenzyl)pyridinium moiety, is stabilized through hydrogen bonding between its nitro group and the reduced cofactor flavin mononucleotide (FMNH
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Registered trials
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