ArticleJournal of microscopy2025
Contrast by electron microscopy in thick biological specimens.
Article in Journal of microscopy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Quantification of Nanovoids in Fully-Aromatic Thin-Film Composite Polyamide Membranes Using a Quartz Crystal Microbalance Equipped with a Humidity Cell.Environmental science & technology · 2026Article
- Electrons and X-rays for diffraction and imaging.Acta crystallographica. Section D, Structural biology · 2026Review
- Damage-limited resolution for X-ray and electron microscopy of organic specimens.Acta crystallographica. Section D, Structural biology · 2026Article
- Contrast by electron microscopy in thick biological specimens.Journal of microscopy · 2025Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The contributions of coherent bright-field phase and incoherent dark-field amplitude contrast are investigated for thick biological specimens. A model for a T4 phage is constructed and images simulated for both TEM and STEM phase contrast using a multislice code. For TEM, the fraction of the illumination intensity available for phase contrast imaging is limited by the fraction of electrons in the zero loss peak, the plasmon peak, or the Landau distribution peak for very thick specimens. These were measured from electron energy loss spectra recorded from various thicknesses of vitreous ice. The incoherent amplitude contrast is simulated using the Penelope Monte Carlo code. Noise limits the features that can be distinguished under the low-dose conditions required for cryo-EM, even for high electron exposures of 100 electrons/Å
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Registered trials
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