Evidence map›Paper›PMID 40856314›Full record

ArticleMolecular pain

Humanized anti-P2X4 scFv reduces ATP-induced P2X4 currents and modulates excitability in human DRG neurons.

Nesia A Zurek, Mark W Shilling, Jenna B Demeter, Reza Ehsanian, Ian M Adams, Aleyah E Goins, Sachin Goyal, Philippe Séguéla, June Bryan I de la Peña, Adinarayana Kunamneni and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular pain. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Nesia A ZurekDepartment of Anesthesiology & Critical Care Medicine, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.ORCID 0009-0003-7939-2886
Mark W ShillingDepartment of Anesthesiology & Critical Care Medicine, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.
Jenna B DemeterDepartment of Anesthesiology & Critical Care Medicine, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.ORCID 0000-0001-5831-9442
Reza EhsanianDepartment of Anesthesiology & Critical Care Medicine, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.
Ian M AdamsDepartment of Anesthesiology & Critical Care Medicine, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.
Aleyah E GoinsDepartment of Anesthesiology & Critical Care Medicine, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.
Sachin GoyalDepartment of Anesthesiology & Critical Care Medicine, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.
Philippe SéguélaDepartment of Neurology and Neurosurgery, Montreal Neurological Institute, Alan Edwards Centre for Research on Pain, McGill University, Montreal, QC, Canada.
June Bryan I de la PeñaDepartment of Anesthesiology & Critical Care Medicine, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.
Adinarayana KunamneniDepartment of Internal Medicine, Mayo Clinic, Jacksonville, FL, USA.
Karin N WestlundDepartment of Anesthesiology & Critical Care Medicine, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.ORCID 0000-0002-7388-4834
Sascha Ra AllesDepartment of Anesthesiology & Critical Care Medicine, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.ORCID 0000-0001-8532-8950

Funding

BLRD VA I01 BX005937
6 · The paper itself

Abstract

Chronic pain affects nearly 100 million adults in the U.S., yet few novel therapeutics have emerged in recent decades. P2X4 receptor (P2X4R), implicated in pain signaling, represents a promising target. We evaluated a humanized single-chain variable fragment (hscFv) targeting P2X4R for its ability to reduce ATP-induced currents and modulate excitability in human dorsal root ganglion (hDRG) neurons. Voltage-clamp recordings confirmed that human P2X4R (hP2X4R) hscFv significantly reduced ATP-evoked currents in HEK-293T cells expressing human P2X4, likely by relocalization of the receptor to the perinuclear region after hscFv treatment. Immunohistochemistry and transcriptomic analyses demonstrated widespread P2X4R (

Indexed as

Adenosine TriphosphateGanglia, SpinalNeuronsReceptors, Purinergic P2X4Single-Chain AntibodiesAction PotentialsAdultFemaleHEK293 CellsHumansMaleMiddle AgedAdenosine TriphosphateReceptors, Purinergic P2X4Single-Chain Antibodieshuman DRGion channelP2X4 receptorPain therapysensory neuronssingle-chain variable fragment antibody (scFv)

Identifiers

PMID40856314
PMCPMC12446818

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.