Evidence map›Paper›PMID 40855411›Full record

ArticleBMC genomics2025

Organic acid accumulation pattern and its key genes in Chinese cherry fruits.

Yan Wang, Jingting Zhou, Xiaocheng Liu, Lisu Hao, Yunfan Yang, Shuaiwei Yang, Hao Wang, Zhenshan Liu, Jing Zhang, Wen He and 8 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Yan Wang *College of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Jingting Zhou *College of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Xiaocheng LiuCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Lisu HaoCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Yunfan YangCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Shuaiwei YangCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Hao WangCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Zhenshan LiuCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Jing ZhangCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Wen HeCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Yuanxiu LinCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Yunting ZhangCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Mengyao LiCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Yong ZhangCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Ya LuoCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Haoru TangCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Qing ChenCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Xiaorong WangCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. wangxr@sicau.edu.cn.

Funding

Sichuan Fruit Innovation Team of National Modern Agricultural Industrial Technology System in China SCCXTD-2024-4Sichuan Science and Technology Program 2024YFHZ0302Sichuan Science and Technology Program 2025ZNSFSC0014
6 · The paper itself

Abstract

backgroundChinese cherry [Cerasus pseudocerasus (Lindl.) G.Don], an economically important fruit species native to southwestern China, plays a key role in regional agriculture. Organic acid composition is crucial for determining organoleptic quality of Chinese cherry, yet the underlying regulatory mechanisms remain unclear.

resultsIn this study, we analyzed organic acid composition in mature fruits from 34 Chinese cherry accessions and tracked dynamic changes throughout fruit development in two landraces with distinct acidity levels (high and low), via HPLC and transcriptomics. Malic acid is the predominant organic acid component, which accounted for 73.66% of total acids. The high-acid landrace exhibited very rapid malate accumulation and relatively fast degradation, while the low-acid landrace showed minor changes in malate levels throughout fruit development. A total of 7,698 DEGs were clustered into six clusters, with DEGs from three clusters being significantly enriched in the "pyruvate metabolism" and "TCA cycle" pathways. Key genes involved in malate biosynthesis (cMDH, PEPC2) and transport (ALMT4, VHP) were up-regulated in the high-acid landrace, while NADP-ME, a gene associated with malate degradation, was down-regulated. Co-expressed network analysis highlighted strong correlations between key structural genes and transcription factors (MYB, ARF, AP2, and bHLH). Notably, CpODORANT1like and CpARF2Blike were identified as potential regulators of acidity, modulating NADP-ME and PEPC2 expression, respectively. These candidate genes were validated through an integrated analysis of phenotypic data and expression pattern of 34 genotypes.

conclusionsOur results suggest that malate accumulation in Chinese cherry is regulated at both the metabolic and vacuolar storage levels. This study deepens our understanding of the mechanisms regulating fruit acidity in Chinese cherry, which could inform future breeding efforts aimed at improving fruit flavor.

Indexed as

AcidsFruitGenes, PlantMalatesChinaGene Expression ProfilingGene Expression Regulation, PlantPlant ProteinsTranscriptomeAcidsMalatesmalic acidPlant ProteinsAccumulation patternChinese cherry fruitsKey genesOrganic acidRegulatory network

Identifiers

PMID40855411
PMCPMC12376409

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LicenceCC BY-NC-ND
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.