Evidence map›Paper›PMID 40850954›Full record

ArticleScientific reports2025

Single-cell transcriptome sequencing reveals tumor stem cells and their molecular characteristics in intrahepatic cholangiocarcinoma.

Xiaowei Gu, Fuzhong Liu, Junfeng Zhang, Nigeerayi Nuermaimaiti, Wenjia Guo, Xiaogang Dong

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Xiaowei GuDepartment of Hepatobiliary and Pancreatic Surgery (Ward 1), Affiliated Cancer Hospital of Xinjiang Medical University, Xinjiang Key Laboratory of Translational Biomedical Engineering, Urumqi, 830000, China.
Fuzhong LiuCancer Research Institute, Affiliated Cancer Hospital of Xinjiang Medical University, Xinjiang Key Laboratory of Translational Biomedical Engineering, Urumqi, 830000, People's Republic of China.
Junfeng ZhangCancer Research Institute, Affiliated Cancer Hospital of Xinjiang Medical University, Xinjiang Key Laboratory of Translational Biomedical Engineering, Urumqi, 830000, People's Republic of China.
Nigeerayi NuermaimaitiCancer Research Institute, Affiliated Cancer Hospital of Xinjiang Medical University, Xinjiang Key Laboratory of Translational Biomedical Engineering, Urumqi, 830000, People's Republic of China.
Wenjia GuoCancer Research Institute, Affiliated Cancer Hospital of Xinjiang Medical University, Xinjiang Key Laboratory of Translational Biomedical Engineering, Urumqi, 830000, People's Republic of China.
Xiaogang DongDepartment of Hepatobiliary and Pancreatic Surgery (Ward 1), Affiliated Cancer Hospital of Xinjiang Medical University, Xinjiang Key Laboratory of Translational Biomedical Engineering, Urumqi, 830000, China. 11418267@zju.edu.cn.

Funding

open project of Xinjiang Uygur Autonomous Region Key Laboratory 2022D04021Tianchi Young Talent Doctoral Program 2022TCYCDXGXinjiang Uygur Autonomous Region Natural Science Foundation 2022D01C290
6 · The paper itself

Abstract

Intercellular communication signals in the tumor microenvironment are closely related to behaviors such as cancer cell proliferation and immune evasion. However, the specific roles of intercellular signaling pathways in intrahepatic cholangiocarcinoma (ICC) have not yet been fully characterized. In this study, we analyzed publicly available single-cell RNA sequencing (scRNA-seq) data derived from paired samples of two intrahepatic cholangiocarcinoma (ICC) tissues and two adjacent normal tissues, thoroughly examining their cellular composition. InferCNV analysis was employed to compare tumor cells and normal cells, and pseudotime analysis was used to identify the growth and differentiation trajectories of the cells. Additionally, intercellular communication analysis was conducted to elucidate the communication networks between cells. Our analysis delineated the cellular ecosystem of ICC, identifying cell subclusters with shared characteristics between ICC and normal tissues. Notably, we characterized a distinct C7-E-T subcluster that exhibited high expression of CXCR4 and BPTF, markers associated with cancer stem cells (CSCs). Further investigation revealed that the MIF intercellular signaling pathway promotes the progression of ICC by activating intracellular signals in the MYC pathway. This study highlights the dysregulation of intercellular signaling pathways within tumor clusters, which influences the onset and progression of ICC. The cancer stem cell subpopulation (CXCR4

Indexed as

Bile Duct NeoplasmsCholangiocarcinomaNeoplastic Stem CellsSingle-Cell AnalysisTranscriptomeBiomarkers, TumorGene Expression ProfilingGene Expression Regulation, NeoplasticHumansReceptors, CXCR4Signal TransductionTumor MicroenvironmentBiomarkers, TumorCXCR4 protein, humanReceptors, CXCR4Cancer stem cellCell communicationIntercellular signaling pathwaysIntrahepatic cholangiocarcinomaSingle-cell

Identifiers

PMID40850954
PMCPMC12375763

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.