ArticleScientific reports2025
Monosodium glutamate enhances freeze-dried mouse sperm quality during room-temperature storage.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Structure-Dependent Triglyceride Protection of Freeze-DriedFoods (Basel, Switzerland) · 2026Article
- Monosodium glutamate protects DNA of freeze-dried somatic cells and improves development of somatic cell nuclear transfer embryos.The Journal of reproduction and development · 2026Article
- The challenges and prospects of mammalian reproduction in extraterrestrial environments.The Journal of reproduction and development · 2026Review
- Freeze-Drying and Room Temperature Preservation of Mammalian Sperm.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Improved birth rates via rehydration of mouse freeze-dried spermatozoa using high-temperature ultrapure water.PloS one · 2026Article
- Hyperosmotic HEPES-CZB Enhances Post-ICSI Survival of C57BL/6N Mouse Oocytes.Reproductive medicine and biologyArticle
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Although freeze-dried (FD) sperm can be stored at room temperature, making it cheaper and safer to store and transport, freeze-drying can damage the sperm's DNA, which can decrease the birth rate compared to frozen sperm. In this study, we examined whether adding monosodium glutamate (MSG), a compound used to dry and preserve yeast, to the drying medium could improve the quality of mouse FD sperm. FD sperm stored using MSG showed lower morphological and DNA damage, and the birth rate of embryos fertilized using those sperm improved to a similar level (50%) to that obtained with frozen-thawed sperm. The offspring showed normal growth and reproductive performance. Similar results were obtained for embryos derived from other mouse strains, even using FD sperm stored for 3 months at room temperature. These results suggest that MSG can protect not only microorganisms but also the DNA of mammalian FD sperm and improve the birth rate to a practical level. This method is not only inexpensive and safer than cryopreservation using liquid nitrogen but has expansion potential to maintain a large number of genetically modified mouse strains.
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