ArticleScientific reports2025
Thymoquinone mediated hedgehog pathway activation and gut microbiota modulation in mouse colitis.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Xu Chunfu's Modified Xianglian Pill Regulates the NOX2/ROS/Mitochondria/NLRP3 Axis to Treat Ulcerative Colitis.Pharmaceuticals (Basel, Switzerland) · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To investigate the treatment of ulcerative colitis by thymoquinone (TQ) through the Hedgehog signaling pathway and the composition of intestinal microbiota. A total of 24 male Balb/C mice were randomly divided into blank group, model group, thymoquinone low-dose group (20 mg/kg) and thymoquinone high-dose group (80 mg/kg), with 6 mice in each group. The concentrations of IL-1β, TNF-α, IL-10 and TGF-β in the supernatant of mouse serum were determined by enzyme-linked immunosorbent assay, and the expressions of Shh, Smo, Gli1, Ptch1, Atresin -1 (ZO-1) and Occludin in the mouse colon were detected by RT-qPCR, Western blot and immunohistochemistry. RNA-seq was used to detect changes in the intestinal microbial community of the four groups at the same time. TQ slowed down body weight, reduced disease activity index, promoted the expression of Shh, Smo, Gli1, Ptch1, Zo-1 and Occuldin in the colon, and increased the phylum levels of Bacteroidota, Campylobacterota, Desulfobacterota, Verrucomicrobiota, Deferribacterota and the abundances of Bacteroidaceae, Muribaculaceae, Akkermansiaceae, Deferrribacteraceae, Staphylococcaceae at the family level, absent from the abundance of Proteobacteria at the phylum level and Enterobacteriaceae at the family level, and inhibited the expression of IL-1β, TNF-α and TGF-β in peripheral blood, and promoted the expression of IL-10 in peripheral blood. Thymoquinone may play a role in the treatment of ulcerative colitis by upregulating Hedgehog and influencing the composition of gut microbiota.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.