Evidence map›Paper›PMID 40847513›Full record

ArticleJournal of food science2025

pH-Dependent Effect of Lecithin on Lupin-Protein-Based Egg Custard Mimics.

Uma Jingxin Tay, Jun Wei Ng, Shiyi Zhang, Daryl Lee, Marco Vignuzzi, Chengxin He, Dingsong Lin, Paolo Alberto Lorenzini, Maria N Antipina, Weibiao Zhou and 1 more

Abstract read
In one paragraph

Article in Journal of food science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Uma Jingxin TayDepartment of Food Science and Technology, Bezos Centre for Sustainable Protein, National University of Singapore, Singapore, Singapore.
Jun Wei NgDepartment of Food Science and Technology, Bezos Centre for Sustainable Protein, National University of Singapore, Singapore, Singapore.
Shiyi ZhangDepartment of Food Science and Technology, Bezos Centre for Sustainable Protein, National University of Singapore, Singapore, Singapore.
Daryl LeeSingapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.
Marco VignuzziInfectious Disease Labs (ID Labs), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.
Chengxin HeDepartment of Food Science and Technology, Bezos Centre for Sustainable Protein, National University of Singapore, Singapore, Singapore.
Dingsong LinDepartment of Food Science and Technology, Bezos Centre for Sustainable Protein, National University of Singapore, Singapore, Singapore.
Paolo Alberto LorenziniInfectious Disease Labs (ID Labs), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.
Maria N AntipinaSingapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.
Weibiao ZhouDepartment of Food Science and Technology, Bezos Centre for Sustainable Protein, National University of Singapore, Singapore, Singapore.
Dejian HuangDepartment of Food Science and Technology, Bezos Centre for Sustainable Protein, National University of Singapore, Singapore, Singapore.ORCID https://orcid.org/0000-0002-2305-3960

Funding

Agency for Science, Technology and Research A21H7a0132Bezos Earth FundNational Research Foundation Singapore NRF2020-THE003-0004
6 · The paper itself

Abstract

This study presents lupin-protein-stabilized emulsions across pH 6.0-8.0, containing 9.92% protein and 7.97% lipid, which formed egg custard mimics upon steaming. To support emulsion stability and potential nutritional benefits, soy lecithin, a phospholipid mixture, was added at 1.0% (w/w) to the emulsion. The effect of lecithin on the gel strength of the mimics was investigated, considering its varying impact across different protein systems. The binding affinity of phosphatidylcholine for β-conglutin across pH 6.0-8.0 was consistent, ranging from -14.89 to -15.53 kcal/mol. At pH 8.0, hydrophobic interactions and hydrogen bonding among proteins were least extensive. Thus, those disrupted by phospholipid complexation were outweighed by those it promoted, both among proteins and between proteins and phospholipids. This resulted in smaller pores, reinforcing the gel strength of the mimic. In contrast, adding lecithin at pH 6.0 and 7.0 reduced hydrogen bonding, which at pH 6.0 also diminished disulfide bonding, ultimately weakening gel strength. As pH influenced protein retention through molecular interactions more strongly than lecithin, retention was highest at pH 6.0 (83.6%) and lowest at pH 8.0 (35.4%), both without lecithin. Correspondingly, these mimics were the strongest and weakest, respectively, based on dynamic consistency index (5.47 vs. 0.783 kPa sⁿ) and breaking force (9.60 vs. 3.81 g). Overall, the mimics were perceived to have gel strength, moisture release, and greasiness comparable to egg custards, though they were grittier and stickier. Besides demonstrating their potential as nutritious egg alternatives, this study offers insight into the textural implications of protein gels incorporating lecithin. PRACTICAL APPLICATIONS: This study presented plant-based egg custard mimics made from lupin-protein-stabilized emulsions, which exhibited textures similar to traditional egg custards. Adding 1.0% soy lecithin altered gel strength in a pH-dependent manner and slightly increased grittiness. These findings on how lecithin affected the texture of lupin-protein emulsion gels provide food companies with practical insights for utilizing lecithin to enhance shelf stability and nutritional benefits in plant-based products.

Indexed as

LecithinsLupinusPlant ProteinsEggsEmulsionsGlycine maxHydrogen BondingHydrogen-Ion ConcentrationHydrophobic and Hydrophilic InteractionsEmulsionsLecithinsPlant Proteins

Identifiers

PMID40847513
PMCPMC12374080

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.