Evidence map›Paper›PMID 40845851›Full record

ArticleStem cell reports2025

XPRESSO: Rapid genetic engineering of human pluripotent stem cells for durable overexpression using a modular anti-silencing vector.

Yehuda Wexler, Harel Grinstein, Irit Huber, Shany Glatstein, Matteo Ghiringhelli, Oded Edri, Michal Landesberg, Daniel Shiff, Gil Arbel, Idan Rosh and 3 more

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Article in Stem cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Yehuda WexlerSohnis Research Laboratory for Cardiac Electrophysiology and Regenerative Medicine, the Rappaport Faculty of Medicine and Research Institute, Technion‒Israel Institute of Technology, POB 9649, Haifa 3109601, Israel.
Harel GrinsteinSohnis Research Laboratory for Cardiac Electrophysiology and Regenerative Medicine, the Rappaport Faculty of Medicine and Research Institute, Technion‒Israel Institute of Technology, POB 9649, Haifa 3109601, Israel.
Irit HuberSohnis Research Laboratory for Cardiac Electrophysiology and Regenerative Medicine, the Rappaport Faculty of Medicine and Research Institute, Technion‒Israel Institute of Technology, POB 9649, Haifa 3109601, Israel.
Shany GlatsteinSohnis Research Laboratory for Cardiac Electrophysiology and Regenerative Medicine, the Rappaport Faculty of Medicine and Research Institute, Technion‒Israel Institute of Technology, POB 9649, Haifa 3109601, Israel.
Matteo GhiringhelliSohnis Research Laboratory for Cardiac Electrophysiology and Regenerative Medicine, the Rappaport Faculty of Medicine and Research Institute, Technion‒Israel Institute of Technology, POB 9649, Haifa 3109601, Israel.
Oded EdriSohnis Research Laboratory for Cardiac Electrophysiology and Regenerative Medicine, the Rappaport Faculty of Medicine and Research Institute, Technion‒Israel Institute of Technology, POB 9649, Haifa 3109601, Israel.
Michal LandesbergSohnis Research Laboratory for Cardiac Electrophysiology and Regenerative Medicine, the Rappaport Faculty of Medicine and Research Institute, Technion‒Israel Institute of Technology, POB 9649, Haifa 3109601, Israel.
Daniel ShiffSohnis Research Laboratory for Cardiac Electrophysiology and Regenerative Medicine, the Rappaport Faculty of Medicine and Research Institute, Technion‒Israel Institute of Technology, POB 9649, Haifa 3109601, Israel.
Gil ArbelSohnis Research Laboratory for Cardiac Electrophysiology and Regenerative Medicine, the Rappaport Faculty of Medicine and Research Institute, Technion‒Israel Institute of Technology, POB 9649, Haifa 3109601, Israel.
Idan RoshSagol Department of Neurobiology Faculty of Natural Sciences, University of Haifa, Haifa 3498838, Israel.
Ashwani ChoudharySagol Department of Neurobiology Faculty of Natural Sciences, University of Haifa, Haifa 3498838, Israel.
Shani SternSagol Department of Neurobiology Faculty of Natural Sciences, University of Haifa, Haifa 3498838, Israel.
Lior GepsteinSohnis Research Laboratory for Cardiac Electrophysiology and Regenerative Medicine, the Rappaport Faculty of Medicine and Research Institute, Technion‒Israel Institute of Technology, POB 9649, Haifa 3109601, Israel; Cardiology Department, Rambam Health Care Campus, 8 Haliya Hasniya St, Haifa 3109601, Israel. Electronic address: mdlior@technion.ac.il.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ectopic expression of proteins in human pluripotent stem cells (hPSCs) is highly desirable as a research tool and important for clinical translation. However, genetically engineering hPSCs for long-term overexpression of proteins remains inefficient, labor-intensive, and plagued by epigenetic silencing, necessitating dedication of significant resources, and entailing laborious workflows. To address these limitations, we report the development of XPRESSO (expedited persistent and robust engineering of stem cells with sleeping beauty for overexpression), a modular "anti-silencing" transposon vector, which we have combined with a highly efficient and accessible methodology for the rapid generation of genetically modified hPSC lines in a gene-independent manner. Using this method, we successfully generated dozens of stable hPSC lines with robust and continuous functional expression of optogenetic proteins, Cas9, shRNA, and a calcium indicator in both undifferentiated and differentiated (cardiomyocyte and neuronal) cells.

Indexed as

Gene SilencingGenetic EngineeringGenetic VectorsPluripotent Stem CellsCell DifferentiationCell LineDNA Transposable ElementsHumansMyocytes, CardiacNeuronsDNA Transposable ElementscardiomyocytesCRISPR-Cas9epigenetic silencinggenetic engineeringhiPSC-CMsneuronsoptogeneticspluripotent stem cellssleeping beauty transposontransgene silencing

Identifiers

PMID40845851
PMCPMC12790730

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.