ArticleStem cell reports2025
Proteomics-based receptor-ligand matching enhances differentiation maturity of human-stem-cell-derived neurons.
Article in Stem cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Growth factor supplementation modulates survival, morphology, and network activity of neurogenin-2 induced human neurons.Scientific reports · 2025Article
- Novel advanced patient-derivedFrontiers in neurologyReview
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6 authors.
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Abstract
Human-induced pluripotent stem cell (hiPSC) technology enables generation of various cell types, offering significant potential for regenerative medicine and personalized disease modeling. However, optimizing the functional maturity of differentiated cells is crucial for improving their reliability in research. Here, we introduce a deep-proteomics-based "receptor-ligand matching" (RLM) strategy to inventory surface receptors on differentiated cells and adjust the culture conditions accordingly. Focusing on an NGN2-induced neuron (iN) model, which rapidly produces glutamatergic neurons but exhibits modest synaptic activity, we identified 3,934 iN membrane proteins, including dozens of growth factor receptors and, notably, the complete GDNF receptor family (GFRA1, GFRA2, and GFRA3) previously undetected. Supplementing culture media with selected ligands enhanced neuronal health, neurite density, and synaptogenesis. Electrophysiology measurements confirmed greater functional synaptic maturity and responsiveness in optimized iNs compared to conventionally generated iNs. The RLM strategy offers a versatile approach to enhance the health and functionality of potentially any hiPSC-derived cell type.
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