Evidence map›Paper›PMID 40845168›Full record

ArticleG3 (Bethesda, Md.)2025

Chromosome-level genome assembly of Dynastes reidi reveals structural evolution of autosomes and the sex chromosomes in Hercules beetles.

My-Hanh Le, Max Proctor, Jen-Pan Huang

Abstract read
In one paragraph

Article in G3 (Bethesda, Md.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

My-Hanh LeBiodiversity Research Center, Academia Sinica, Taipei 11529, Taiwan.
Max ProctorDivision of Biological Sciences, University of Montana, Missoula, MT 59812, United States.
Jen-Pan HuangBiodiversity Research Center, Academia Sinica, Taipei 11529, Taiwan.ORCID 0000-0002-9329-8867

Funding

Academia Sinica AS-PD-11202-L09National Science and Technology Council NSTC 114-2621-B-001-006-MY3Taiwan International Internship Program
6 · The paper itself

Abstract

The Hercules beetles have long been iconic symbols of evolutionary diversification, sexual selection, and systematics. Despite their rapid phenotypic evolution and a rich history of inspiring evolutionary biologists, genomic resources for these charismatic beetles remain limited, especially for the Giant Hercules beetles. We present the first chromosome-level genome assembly of a Giant Hercules beetle from the Lesser Antilles. The assembled genome is approximately 837 Mb in size, with a scaffold N50 of 66.68 Mb, which can be anchored to 11 pseudochromosomes with a BUSCO completeness score of 95.9%. An estimate of 55.5% of the genome can be attributed to repetitive elements. Additionally, we detected candidate sex-linked chromosomes by comparing sequencing read depths between 1 male and 2 females using Illumina short reads. The chromosome-level genome assembly of Dynastes reidi not only provides critical insights into evolutionary and functional genomics but also supports informed conservation and management efforts. In addition, this genomic resource will enable future pangenome analyses aimed at understanding the genetic basis of species divergence and morphological innovation in beetles. Our study also marks the emergence of a new model system to investigate the origin and diversification of phenotypic novelty by leveraging genomic resources across diverse domesticated beetle breeds.

Indexed as

Chromosomes, InsectColeopteraEvolution, MolecularGenome, InsectSex ChromosomesAnimalsFemaleGenomicsMaleMolecular Sequence AnnotationDynastes reidigenome assemblyGiant Hercules beetlesex chromosome identificationsex-linked region

Identifiers

PMID40845168
PMCPMC12611238

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.