ArticlePloS one2025
Comparison of the composition and antiplasmodial activity of Artemisia annua teas using an untargeted metabolomic approach.
Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Phytochemical synergy in artemisia annua herbal tea against malaria: a systematic review of its efficacy and safety in the context of emerging Pfkelch13 resistance.Infectious diseases of poverty · 2026Pooled it
- Cadinapyridine sesquiterpene alkaloids from Artemisia annua and in vitro cytotoxicity and antiplasmodial activities.PloS one · 2025Article
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Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of teas made from locally cultivated Artemisia annua to fight malaria in remote areas where access to care is difficult is a matter of debate. This study aimed at document differences in the composition of A. annua teas cultivated in Benin to be sold as antimalarial teas, and in France, and their impact on antiplasmodial activity. A. annua teas were prepared with plants from one location in south France and from ten different plantations in Benin. Artemisinin was quantified in herbal teas with a liquid chromatography system coupled to mass spectrometry and multiple reaction monitoring detection methods. The herbal teas were tested against chloroquine-sensitive 3D7 strain of Plasmodium falciparum using isotopic microtest to determine IC50 values and calculate the concentration of artemisinin corresponding to the IC50 of the teas [ART(tea)_IC50]. Chemical profiles were determined by liquid chromatography coupled to high resolution mass spectrometry and a metabolomic analysis was performed to annotate compounds statistically linked to the antiplasmodial properties of the teas. Artemisinin content varied between 0.3 mg/L for tea with plants from France to 15.7 mg/L for teas made with plants from Benin with differences between locations. Artemisinin content was decreasing after a one-year storage of the plant for 3 localities in Benin with loss of 33%, 48% and 24% (P < 0.05). Artemisinin concentrations and antiplasmodial activity of teas were positively correlated although the comparison of ART(tea)_IC50s to IC50 of pure artemisinin suggested that other compounds present in the tea were involved in the activity, either enhancing or limiting it. Unknown alkaloids in A. annua teas correlated to antiplasmodial activity were also detected. These findings suggest that A. annua teas deserve further studies to identify other metabolites of interest and determine their role in antiplasmodial activity in relation to other molecules, particularly artemisinin.
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