Evidence map›Paper›PMID 40844876›Full record

ArticleCell reports2025

A consensus definition for deep layer 6 excitatory neurons in mouse somatosensory, visual, and motor cortex.

Su-Jeong Kim, Travis A Babola, Kiwoong Lee, Alina C Spiegel, Kihwan Lee, Chanel J Matney, Michael H Liew, Eva M Schulteis, Austin E Coye, Mikhail Proskurin and 11 more

Abstract readConsensus Statement
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

21 authors.

Su-Jeong KimSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Travis A BabolaSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Kiwoong LeeSchool of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea; Emotion, Cognition and Behavior Research Group, Korea Brain Research Institute, Daegu 41062, Republic of Korea.
Alina C SpiegelSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Kihwan LeeSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Chanel J MatneySolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Michael H LiewSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Eva M SchulteisSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Austin E CoyeSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Mikhail ProskurinSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Hyunwook KangSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Julia A KimSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Maxime ChevéeSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Juhyun LeeSchool of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea; Emotion, Cognition and Behavior Research Group, Korea Brain Research Institute, Daegu 41062, Republic of Korea.
Alec G CabalinanSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Seong Yeol AnSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Samuel P BronckersSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Patrick O KanoldKavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Loyal A GoffSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; McKusick-Nathans Institute for Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Juhyun KimSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Emotion, Cognition and Behavior Research Group, Korea Brain Research Institute, Daegu 41062, Republic of Korea; Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address: juhyun.kim@kbri.re.kr.
Solange P BrownSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address: spbrown@jhmi.edu.

Funding

Medical Scientist Training ProgramT32GM136577 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI ANDREA L COX · 2020 to 2026
$13.4M
Murine Mutagenesis CoreP30NS050274 · NINDS · JOHNS HOPKINS UNIVERSITY · PI KOLODKIN, ALEX L · 2005 to 2020
$10.9M
NEURONAL CIRCUITS AND PLASTICITY OF NEONATAL AUDITORY CORTEXR01DC009607 · NIDCD · UNIV OF MARYLAND, COLLEGE PARK · PI PATRICK O KANOLD · 2009 to 2026
$7.8M
Neuroscience Training ProgramT32EY017203 · NEI · JOHNS HOPKINS UNIVERSITY · PI BERGLES, DWIGHT E · 2005 to 2014
$2.9M
The functional organization of the claustrumR01NS085121 · NINDS · JOHNS HOPKINS UNIVERSITY · PI BROWN, SOLANGE P · 2017 to 2020
$1.4M
The functions of the claustrum in flexible decision making.R56MH138416 · NIMH · JOHNS HOPKINS UNIVERSITY · PI BROWN, SOLANGE P · 2025 to 2025
$608k
Layer 6b, a novel inhibitory gain controller in the neocortexR21MH128765 · NIMH · JOHNS HOPKINS UNIVERSITY · PI KAMIYA, ATSUSHI · 2022 to 2023
$450k
NEI NIH HHS T32 EY017203NIDCD NIH HHS R01 DC009607NIGMS NIH HHS T32 GM136577NIMH NIH HHS R21 MH128765NIMH NIH HHS R56 MH138416NINDS NIH HHS P30 NS050274NINDS NIH HHS R01 NS085121
6 · The paper itself

Abstract

To understand neocortical function, it is helpful to define cortical cell types. Recent studies indicate that neurons in the deepest cortical layer play roles in mediating thalamocortical interactions and modulating brain state and are implicated in neuropsychiatric disease. However, understanding the functions of deep layer 6 (layer 6b [L6b]) neurons has been hampered by the lack of agreed-upon definitions for these neuron types. We compared commonly used methods for defining L6b neurons, including molecular, transcriptional, and morphological approaches and transgenic mouse lines, and identified a core population of L6b neurons. This population does not innervate the thalamus, unlike layer 6 corticothalamic neurons (L6CThNs) in more superficial layer 6. Rather, single L6b neurons project ipsilaterally between cortical areas. Their intrinsic electrophysiological properties were stable after the first two postnatal weeks. The four methods we identify for defining L6b neurons enable comparisons across studies testing their contributions to cortical function.

Indexed as

Motor CortexNeuronsSomatosensory CortexVisual CortexAnimalsMaleMiceMice, Inbred C57BLMice, TransgenicThalamuscorticothalamic neuronsCplx3CP: NeuroscienceCTGFDrd1alayer 6bmotor cortexNxph4somatosensory cortexthalamusvisual cortex

Identifiers

PMID40844876
PMCPMC12505899

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.