Evidence map›Paper›PMID 40844648›Full record

ArticleVeterinary medicine and science2025

Comparative Detection and Genetic Characterization of Feline Panleukopenia Virus in Bangladesh.

Nurejunnati Jeba, Roni Mia, Md Masum Billah Tarafder, Anandha Mozumder, Raduyan Farazi, S M Nazmul Hasan, Mohammad Bayazid Bostami, A K M Anisur Rahman, Abdul Mannan, Sharmin Akter and 5 more

Abstract readComparative Study
PubMed Full text at PMC Publisher
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Article in Veterinary medicine and science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

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0cells of the map it votes in
3citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Nurejunnati JebaDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Roni MiaDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Md Masum Billah TarafderDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Anandha MozumderDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Raduyan FaraziDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
S M Nazmul HasanDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Mohammad Bayazid BostamiTeaching and Training Pet Hospital and Research Center, Chattogram Veterinary and Animal Sciences University, Chattogram, Bangladesh.
A K M Anisur RahmanDepartment of Medicine, Bangladesh Agricultural University, Mymensingh, Bangladesh.ORCID https://orcid.org/0000-0001-9660-4949
Abdul MannanTeaching and Training Pet Hospital and Research Center, Chattogram Veterinary and Animal Sciences University, Chattogram, Bangladesh.
Sharmin AkterDepartment of Physiology, Bangladesh Agricultural University, Mymensingh, Bangladesh.ORCID https://orcid.org/0000-0001-6901-3377
Sukumar SahaDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.ORCID https://orcid.org/0000-0002-8036-0190
Tofazzal IslamInstitute of Biotechnology and Genetic Engineering, Gazipur Agricultural University, Gazipur, Bangladesh.
Elcio LealInstitute of Biological Sciences, Federal University of Pará, Belem, Pará, Brazil.
Antonio Charlys da CostaInstituto de Medicina Tropical, Faculdade de Medicina, Universidade de São Paulo, São Paulo, State of São Paulo, Brazil.
Md Golzar HossainDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.ORCID https://orcid.org/0000-0002-1487-5444

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundFeline panleukopenia virus (FPV) is a highly contagious and often fatal disease affecting domestic and wild felines. Accurate diagnosis and understanding of circulating strains are essential for effective control.

objectivesThis study aimed to evaluate the diagnostic accuracy of a rapid immunochromatographic (IC) antigen test compared to PCR for FPV detection in clinically suspected pet cats in Bangladesh. It also aimed to investigate the genetic and evolutionary characteristics of circulating FPV strains.

methodsFaecal or rectal swab samples from suspected cats were tested using both IC strip tests and PCR. Sensitivity and specificity of the IC test were analysed using PCR as the reference. Partial sequencing of the VP2 gene was performed on four PCR-positive samples for phylogenetic and mutational analysis. Structural modelling of VP2 proteins was conducted to predict conformational changes.

resultsThe IC test detected FPV in 84% of cases, whereas PCR confirmed only 60%, indicating a 24% false-positive rate. PCR showed higher diagnostic reliability. FPV prevalence was 92% among unvaccinated cats. Phylogenetic analysis of VP2 sequences revealed close genetic similarity with Chinese and Portuguese strains, suggesting possible cross-border transmission. Mutations such as A756G, A896G, E299G and T236I were consistently observed. Structural modelling indicated minor conformational changes in VP2. CONCLUSION AND CLINICAL SIGNIFICANCE: PCR offers superior accuracy over IC testing for FPV diagnosis. Mutational changes may impact antigenicity and diagnostic performance. Improved diagnostic accuracy, molecular surveillance and updated vaccination strategies are essential to control FPV outbreaks in feline populations.

Indexed as

Feline PanleukopeniaFeline Panleukopenia VirusPolymerase Chain ReactionAnimalsBangladeshCatsPhylogenySensitivity and SpecificityFeline panleukopenia virusIC strip testPCRphylogenetic analysisVP2 gene mutations

Identifiers

PMID40844648
PMCPMC12372610

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