Evidence map›Paper›PMID 40842983›Full record

ArticleFrontiers in immunology2025

Recombinant SADS-CoV as a vector for porcine epidemic diarrhea vaccine development.

Xiaoling Yan, Xiaoli Zhang, Xiaocheng Lyu, Yaoyao Zheng, Qianniu Li, Xiaoya Zhao, Jun Fu, Jingyun Ma

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xiaoling Yan *State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou, China.
Xiaoli Zhang *State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou, China.
Xiaocheng LyuState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou, China.
Yaoyao ZhengState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou, China.
Qianniu LiState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou, China.
Xiaoya ZhaoState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou, China.
Jun FuState Key Laboratory of Microbial Technology, Shandong University-Helmholtz Institute of Biotechnology, Shandong University, Qingdao, China.
Jingyun MaState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Swine acute diarrhea syndrome coronavirus (SADS-CoV) is an emerging porcine enteric coronavirus that can cause diarrhea in piglets younger than 5 days of age. However, infection of pigs older than 5 days of age does not usually result in obvious clinical symptoms. This relative intrinsic safety in older animals prompted us to investigate the potential of SADS-CoV as a viral vector for porcine diarrhea virus vaccines. Methods: We utilized Gibson assembly to clone the SADS-CoV sequence into an artificial bacterial chromosome (BAC) vector. Further manipulation was carried out by recombineering to generate four attenuated recombinant SADS-CoV strains expressing a PEDV protective antigen fused with peptides that target immune cells. Subsequently, the biological characteristics and immunogenic efficacy of these four recombinant strains were systematically assessed through Results and discussion: The recombinant viruses exhibited a proliferation profile similar to that of the wild-type virus in Vero cells, maintained stable cytopathic effects, retained the exogenous sequences for up to 20 passages, and consistently expressed the PEDV antigen fusion protein. Immunizing pregnant sows with these recombinant viruses effectively enhanced both cellular and mucosal immune responses and provided significant clinical protection against PEDV to their offspring. This study not only generated a vaccine candidate for PEDV but also established a pipeline for using the SADS-CoV as a vector for vaccine development.

Indexed as

Coronavirus InfectionsGenetic VectorsPorcine epidemic diarrhea virusSwine DiseasesVaccine DevelopmentViral VaccinesAlphacoronavirusAnimalsAntibodies, ViralChlorocebus aethiopsFemalePregnancySwineVero CellsAntibodies, ViralViral Vaccinesattenuationporcine epidemic diarrhea virus (PEDV)reverse geneticsswine acute diarrhea syndrome coronavirusviral vector vaccines

Identifiers

PMID40842983
PMCPMC12364652

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.