ArticleFrontiers in cellular and infection microbiology2025
Exploring protein-protein ligation approaches for the cytosolic delivery of antigens using AIP56.
Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: The intracellular delivery of biologics, particularly large cargoes like proteins, remains a challenge in biotechnology and biomedicine. The modular structure of well-characterized AB toxins allows different cargoes to be grafted, creating a target-specific biotechnological tool capable of cytosolic delivery. Methods: In this study, we employed protein-protein fusion strategies-SpyCatcher003, SnoopCatcher, and SnoopLigase-to generate chimeras between the delivery region of AIP56 (AIP56 Results: The chimeras were successfully obtained using these strategies and were all able to deliver β-lactamase into the cytosol of J774.A1 macrophages. Cellular fractionation showed that, although most of the β-lactamase remains associated with the endosomal compartment, an active portion is released into the cytosol. Conclusion: AIP56 delivery region transporting other cargo directly to the cytosol of antigen-presenting cells might be a promising platform for antigen/cargo delivery. This study highlights the potential of protein-protein fusion strategies to create versatile, antigenically distinct toxin-based delivery systems for therapeutic applications.
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