ArticleFrontiers in genetics2025
High-throughput PacBio library preparation and sequencing techniques for genomic DNA and TNA.
Article in Frontiers in genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- Proteogenomics: decoding cancer in multiple dimensions.MedScience · 2026Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PacBio Sequencing is an effective tool for achieving long read lengths to close gaps in whole genome assemblies. Limited information is available for automated, high-throughput library preparation workflows for PacBio sequencing. This paper describes two high throughput methods, one for producing barcoded genomic DNA libraries and one for barcoded cDNA and subsequent library preparation, as well as pooling and sequencing on the Sequel II instrument. Test sequencing was performed on 380 genomic DNA samples, of which 28 (7.4%) failed sequencing and needed to be repeated. Test sequencing was performed on 384 Threose nucleic acid samples of which 0 failed sequencing and 49 (12.8%) had a genome coverage <90%. These methods were used to generate and report results for ∼2,000 genomic DNA and ∼30,000 cDNA genome sequences from clinical specimens in 2023.
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Registered trials
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