ArticleMicrobial cell factories2025
Regulation of the Ald gene encoding alanine dehydrogenase and its induction of ammonium-tolerant nitrogen fixation in Paenibacillus polymyxa WLY78.
Article in Microbial cell factories, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
backgroundPaenibacillus polymyxa WLY78, a Gram-positive diazotroph with plant growth promotion and phytopathogen suppression, represents a promising candidate for agricultural biofertilizers. However, its nitrogen fixation capacity is inherently limited by ammonium-mediated repression. Recent studies revealed that ammonium-tolerant nitrogen fixation in certain Paenibacillus species correlates with alanine overproduction mediated by alanine dehydrogenase (ADH) encoded by the ald gene.
resultsThis study establishes a dual regulatory mechanism governing ald expression in P. polymyxa WLY78. The transcription activator AdeR positively regulates ald expression, while the global nitrogen regulator GlnR exerts repression on both ald and its activator gene adeR. Under high ammonium conditions, GlnR-mediated suppression maintains basal ald expression levels, preventing alanine biosynthesis. Upregulation of ald expression through high-copy plasmid or mutagenesis of GlnR-binding sites in the adeR-ald regulatory region significantly enhanced alanine concentration. Both endogenous overproduction and exogenous supplementation of alanine suppressed glutamine synthetase (GS) activity, thereby reducing intracellular glutamine levels. This prevents the formation of glutamine-feedback-inhibited GS complexes (FBI-GS), disrupting the GlnR-FBI-GS interaction required for nif gene repression. Consequently, GlnR transitions to its activated state, enabling nif gene expression even under elevated ammonium concentrations.
conclusionsOur findings elucidate a conserved regulatory paradigm in Paenibacillus species where alanine metabolism modulates nitrogen fixation through GS-mediated metabolic signaling. The ald overexpression or exogenous alanine supplementation can bypass ammonium inhibition provides practical strategies for enhancing biofertilizer performance in nitrogen-rich agricultural soils.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.