ArticleCommunications chemistry2025
Membrane charge primes the necroptotic kinase RIPK3 for amyloid assembly.
Article in Communications chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
3 citing papers in PubMed.
- Functional amyloids as molecular switches: emerging roles in diverse signalling pathways.Biochemical Society transactions · 2026Review
- Charged membrane interfaces reshape the nucleation landscape of RIPK3 amyloid variants.European biophysics journal : EBJ · 2026Article
- Solution NMR reveals micelle-stabilized α-helical segments flanking the RIPK1 RHIM amyloid core.Biochemical and biophysical research communications · 2026Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Receptor-interacting protein kinase 3 (RIPK3) drives necroptosis by assembling into functional amyloid fibrils. Here we show that lipids modulate RIPK3 amyloidogenesis by stabilizing an aggregation-prone intermediate. While electrostatic repulsion maintains RIPK3 in a soluble state, charge compensation alone is not sufficient for fibril formation and hydrophobic contacts are required to initiate nucleation. Using solution-state NMR, fluorescence-based assays and polymer-encased lipid particles, we demonstrate that negatively charged membranes selectively recruit RIPK3 and restrict its conformational flexibility, accelerating aggregation. These findings reveal a membrane-guided mechanism for RIPK3 assembly and suggest that lipid surfaces, like those implicated in pathological amyloid formation, may modulate functional amyloidogenesis even in the absence of canonical necroptotic stimuli.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.