ArticleBMC plant biology2025
Characterization and transcriptomic analysis provide novel insights into the sexual differentiation of the dioecious plant Bama Huoma (Cannabis sativa).
Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Transcriptomic insights into unisexual flower development in Cannabis sativa: conserved MADS-box networks and divergent developmental programs.BMC plant biology · 2026Article
- Correction: Characterization and transcriptomic analysis provide novel insights into the sexual differentiation of the dioecious plant Bama Huoma (Cannabis sativa).BMC plant biology · 2025Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors.
Funding
Abstract
backgroundBama Huoma is a high-value cash crop because its seeds are known as the "holy seeds of longevity". However, its seed yield is restricted by dioecism, and information about flower sex differentiation in this plant is lacking.
resultsIn this study, anatomical analysis and dynamic transcriptome profiling were performed to explore the mechanism of sex differentiation in Bama Huoma. The results revealed that female and male flowers undergo independent developmental processes and that sex differentiation occurs at an early stage before flower development. Illumina sequencing revealed that MADS-box genes play crucial roles in pistil and stamen development. qRT‒PCR and RNA in situ hybridization were subsequently performed to determine the spatiotemporal expression patterns of genes related to sex determination. The results revealed that eight MADS-box genes and two MYB genes were expressed in a sex-biased and tissue-specific manner in male or female flowers. Among these genes, MYB35, PMADS2, TM6 and AGL104 were specifically expressed in male flowers and found to regulate callose metabolism, pollen sac formation, tapetum development, and microspore maturation. In contrast, AGL11, FBP24, AGL16 and two CAL were restricted to female flowers, where they regulate pistil formation, ovary development, and stigma differentiation. These genes play distinct and essential roles in the sex differentiation of Bama Huoma, as well as in the development of floral organs.
conclusionOur findings provide a comprehensive gene expression profile of flower sex differentiation in Bama Huoma as well as new clues and information for further study in this field.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.