Evidence map›Paper›PMID 40838359›Full record

ArticleAdvanced healthcare materials2026

Myeloperoxidase Luminol Reaction - A Novel Faecal Assay for Predicting Colonoscopy Findings in Patients with Ulcerative Colitis: A Pilot Cross-Sectional Clinical Study.

Viraj Kariyawasam, Taylor Davis, Mark Ghali, David Tejcek, Thomas R O'Neil, Ella Verley, Matthew Griffiths, Miguel Campos, Ingrid Bonney, Andrew C Bulmer and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced healthcare materials, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Viraj KariyawasamBlacktown/Mt. Druitt Clinical School, University of Western Sydney, Sydney, NSW, 2148, Australia.
Taylor DavisRedox Inflammation Group, Charles Perkins Centre, School of Medical Sciences, Faculty of Medicine & Health, The University of Sydney, Sydney, NSW, 2006, Australia.
Mark GhaliBlacktown/Mt. Druitt Clinical School, University of Western Sydney, Sydney, NSW, 2148, Australia.
David TejcekBiomedical Engineering, Faculty of Engineering, The University of Sydney, Sydney, NSW, 2006, Australia.
Thomas R O'NeilThe Westmead Institute for Medical Research, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Westmead, Sydney, NSW, 2145, Australia.
Ella VerleyRedox Inflammation Group, Charles Perkins Centre, School of Medical Sciences, Faculty of Medicine & Health, The University of Sydney, Sydney, NSW, 2006, Australia.
Matthew GriffithsBiomedical Engineering, Faculty of Engineering, The University of Sydney, Sydney, NSW, 2006, Australia.
Miguel CamposBiomedical Engineering, Faculty of Engineering, The University of Sydney, Sydney, NSW, 2006, Australia.
Ingrid BonneyBiomedical Engineering, Faculty of Engineering, The University of Sydney, Sydney, NSW, 2006, Australia.
Andrew C BulmerSchool of Pharmacy and Medical Sciences, Griffith University, Gold Coast, QLD, 4222, Australia.
Yogambha RamaswamyBiomedical Engineering, Faculty of Engineering, The University of Sydney, Sydney, NSW, 2006, Australia.
Nikola MitrevBlacktown/Mt. Druitt Clinical School, University of Western Sydney, Sydney, NSW, 2148, Australia.
Crispin CorteAW Morrow Gastroenterology and Liver Centre, Royal Prince Alfred Hospital, Sydney, NSW, 2050, Australia.
Paul K WittingRedox Biology Group, Charles Perkins Centre, School of Medical Sciences, Faculty of Medicine & Health, The University of Sydney, Sydney, NSW, 2006, Australia.
Belal ChamiRedox Inflammation Group, Charles Perkins Centre, School of Medical Sciences, Faculty of Medicine & Health, The University of Sydney, Sydney, NSW, 2006, Australia.ORCID 0000-0002-8491-8614

Funding

Crohn's and Colitis Foundation 827399Faculty of Medicine and Health, University of Sydney CT31847
6 · The paper itself

Abstract

Inflammatory Bowel Disease (IBD), including ulcerative colitis (UC), requires non-invasive, convenient and cost-effective biomarkers for disease monitoring. Myeloperoxidase (MPO), a neutrophil-derived enzyme, correlates with intestinal inflammation. This study aims to assess the Myeloperoxidase Luminol Reaction (MPOLR) assay - a novel chemiluminescent-based method for detecting faecal MPO (fMPO) activity, to improve the prediction of endoscopic findings in UC. A cross-sectional clinical study recruited 39 participants, categorized into UC (n = 20), colonoscopy control (CC; n = 9), and healthy control (HC; n = 10). Faecal samples are analysed for fMPO, calprotectin (fCalpro), and lactoferrin (fLacto) using ELISA and the MPOLR assay. The MPOLR assay is validated against endoscopic disease severity (UCEIS), symptomology, and clinical indices. MPOLR strongly correlated with UCEIS (ρ = 0.78), disease severity (ρ = 0.75), and symptomology (ρ = 0.78), outperforming fCalpro (ρ = 0.58, ρ = 0.57, and ρ = 0.41, respectively). AUROC analysis reveals MPOLR (0.78) and fMPO (0.75) have superior predictive potential for UC diagnosis compared to fCalpro (0.66). MPOLR is a rapid, cost-effective assay that enhances UC monitoring by accurately reflecting endoscopic findings and outcompetes fCalpro and other faecal biomarkers' predictive potential for UC diagnosis.

Indexed as

Colitis, UlcerativeColonoscopyFecesLuminolPeroxidaseAdultAgedBiomarkersCross-Sectional StudiesFemaleHumansLeukocyte L1 Antigen ComplexMaleMiddle AgedPilot ProjectsBiomarkersLeukocyte L1 Antigen ComplexLuminolPeroxidaseCalprotectinChemiluminescenceInflammatory Bowel DiseaseLactoferrinMyeloperoxidaseUlcerative colitis

Identifiers

PMID40838359
PMCPMC12790305

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.