ArticleFrontiers in microbiology2025
Development of a capillary-modified naked-eye visual loop-mediated isothermal amplification method for the rapid detection of mpox virus and chikungunya virus.
Article in Frontiers in microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Naked-Eye Visual Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Chikungunya Virus.Methods in molecular biology (Clifton, N.J.) · 2027Article
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7 authors.
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Abstract
The global emergence of mpox virus (MPXV) and chikungunya virus (CHIKV) has intensified the demand for advanced diagnostic methods. Rapid, sensitive, cost-effective diagnostic methods are crucial for preventing cross-border transmission and early containment of community spread. In this study, we developed a capillary modified Loop-mediated isothermal amplification (LAMP) assay for the identification of MPXV and CHIKV. This system employs capillaries as reaction vessels, offering advantages such as reduced reagent consumption and simplified operation. The capillary-driven liquid handling system also significantly reduces the frequency of lid openings during reagent transfer compared to standard LAMP protocols. This minimizes the risks of aerosol contamination and the associated false-positive outcomes that are inherent to conventional methods. Additionally, direct visual interpretation of the results without specialized instrumentation is achieved through integration of a leuco-hydroxynaphthol blue (LHNB) dye. This novel detection method targets the F13 gene of MPXV, the nsP1 gene of CHIKV, live vaccinia virus (VACV) and CHIKV viruses. Analytical sensitivity reached 10 copies/μL for MPXV F13 and 6 copies/μL for CHIKV nsP1. Because of the high level of laboratory biosafety required for MPXV culture, VACV was selected as a safe surrogate model for detection, where the E9L gene was selected to target all
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