ArticleBiochemistry and biophysics reports2025
Deciphering tryptophan metabolism in colorectal cancer through multi-omics analysis.
Article in Biochemistry and biophysics reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Tryptophan Metabolism in Digestive and Extra-Digestive Diseases: Mechanisms, Clinical Implications, and Therapeutic Perspectives.Nutrients · 2026Review
- Integrative single-cell and multi-omics analysis of ZBTB21-mediated serine metabolism in colorectal cancer: from metabolic reprogramming to immune microenvironment modulation.Cancer immunology, immunotherapy : CII · 2026Article
- Regulation of PD-1PD-L1 Immune Checkpoints by Gut Microbiota Metabolites and Their Clinical Translational Research: A Review.Immunity, inflammation and disease · 2026Review
- Review
- Metabolomics and proteomics analyses reveal the potiental mechanisms underlying colorectal cancer and chemotherapy targets.BMC cancer · 2026Article
- Investigating tryptophan metabolism in colorectal cancer using Single-cell RNA sequencing based on machine learning techniques.PloS one · 2026Article
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Authors and funding
15 authors.
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Abstract
Metabolic reprogramming is essential for colorectal cancer (CRC) progression, and recent studies have pointed to tryptophan metabolism as a crucial modulator of the tumor immune microenvironment. In this study, we performed untargeted metabolomics analyses and identified significant differences between CRC tissues and matched adjacent tissues, highlighting alterations in tryptophan metabolism. Targeted metabolomics, combined with public single-cell RNA sequencing datasets, further validated enhanced tryptophan metabolism activity in CRC, correlating closely with tumor purity and poor patient prognosis. Using multiple machine learning algorithms, we developed and validated a prognostic risk model based on key tryptophan metabolism-related genes across several independent cohorts. Single-cell transcriptomic analyses also revealed a distinct tumor cell subcluster (C1), characterized by elevated tryptophan metabolism and associated with tumor progression. Additionally, increased tryptophan metabolism correlated positively with M2 macrophage infiltration, and our in vitro co-culture assays confirmed that CRC cell-derived tryptophan metabolites could directly induce M2 macrophage polarization. Together, these results indicate that tryptophanmetabolism is pivotal in CRC development and immune escape, presenting potential novel targets for therapeutic intervention.
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