Evidence map›Paper›PMID 40836925›Full record

ArticleiScience2025

Inducible NMDA receptor knockdown reveals a maintenance phase in dendritic refinement of barrel cortex neurons.

Ayane Nihashi, Naoki Nakagawa, Takuya Sato, Mariko Yamamoto, Luwei Wang, Rieko Ajima, Yumiko Saga, Yumiko Yoshimura, Masato T Kanemaki, Takuji Iwasato

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In one paragraph

Article in iScience, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Ayane NihashiLaboratory of Mammalian Neural Circuits, National Institute of Genetics, Mishima, Japan.
Naoki NakagawaLaboratory of Mammalian Neural Circuits, National Institute of Genetics, Mishima, Japan.
Takuya SatoLaboratory of Mammalian Neural Circuits, National Institute of Genetics, Mishima, Japan.
Mariko YamamotoDivision of Visual Information Processing, National Institute for Physiological Sciences, Okazaki, Japan.
Luwei WangLaboratory of Mammalian Neural Circuits, National Institute of Genetics, Mishima, Japan.
Rieko AjimaDivision of Embryology, National Institute for Basic Biology, Okazaki, Japan.
Yumiko SagaMammalian Development Laboratory, National Institute of Genetics, Mishima, Japan.
Yumiko YoshimuraDivision of Visual Information Processing, National Institute for Physiological Sciences, Okazaki, Japan.
Masato T KanemakiGraduate Institute for Advanced Studies, SOKENDAI, Mishima, Japan.
Takuji IwasatoLaboratory of Mammalian Neural Circuits, National Institute of Genetics, Mishima, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The temporal mechanisms of activity-dependent dendritic patterning during postnatal development remain unclear because appropriate technology is lacking. Here, we demonstrate that the auxin-inducible degron 2 technology enables the rapid knockdown of target proteins at specific time points in the postnatal mouse brain. When N-methyl-D-aspartate-type glutamate receptor (NMDAR) depletion was induced from postnatal day (P)3, barrel cortex layer 4 spiny stellate neurons (barrel cells) failed to form strong asymmetry and a high tree-length variance in the dendritic patterns. Intriguingly, these unique dendritic patterns of barrel cells formed by P6 were rapidly canceled by NMDAR depletion from P6 but not from P12. NMDAR depletion from P6 also extinguished the existing Golgi apparatus' lateral polarity. These results suggest that the barrel cells' dendritic refinement involves not only formation but also "maintenance" of specific dendritic patterns during early postnatal development, in which NMDARs play a critical role, likely by regulating the Golgi polarity.

Indexed as

Molecular biologyNeuroscience

Identifiers

PMID40836925
PMCPMC12361620

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.