Evidence map›Paper›PMID 40835937›Full record

ArticleBMC research notes2025

Evaluating the recovery of pan-susceptible and antibiotic-resistant Escherichia coli in synthetic test agricultural water using membrane filtration and colilert methods.

Zirui Ray Xiong, Ajani A Brooks, Ellen Gabriel, Alan Gutierrez, Shayla B Johnson, Cheryl East, Lisa M Durso, Elica Moss, Manan Sharma

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC research notes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Zirui Ray XiongUnited States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Environmental Microbial and Food Safety Laboratory, Beltsville, MD, United States of America.
Ajani A BrooksUnited States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Environmental Microbial and Food Safety Laboratory, Beltsville, MD, United States of America.
Ellen GabrielUnited States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Environmental Microbial and Food Safety Laboratory, Beltsville, MD, United States of America.
Alan GutierrezUnited States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Environmental Microbial and Food Safety Laboratory, Beltsville, MD, United States of America.
Shayla B JohnsonUnited States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Environmental Microbial and Food Safety Laboratory, Beltsville, MD, United States of America.
Cheryl EastUnited States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Environmental Microbial and Food Safety Laboratory, Beltsville, MD, United States of America.
Lisa M DursoUnited States Department of Agriculture, Agricultural Research Service, Agroecosystems Management Research, Lincoln, NE, United States of America.
Elica MossDepartment of Plant and Soil Science, Alabama A&M University, Huntsville, AL, United States of America.
Manan SharmaUnited States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Environmental Microbial and Food Safety Laboratory, Beltsville, MD, United States of America. manan.sharma@usda.gov.

Funding

U.S. Department of Agriculture, Agricultural Research Service 8042-42610-001-000-D
6 · The paper itself

Abstract

Membrane filtration and Colilert assays are commonly used to quantify Escherichia coli levels in agricultural water. These methods have not been evaluated in test agricultural water (TAW), a formulation used for assessing chemical sanitizer effectiveness. Quantitative recovery of E. coli in TAW may be affected by turbidity levels. TAW was formulated with a pH value of 6.5 at two turbidity levels (0 and 50 NTU). Pan-susceptible and cefotaxime-resistant E. coli strains were added separately to TAW (200 CFU/100 mL). Inoculated TAW was either (1) filtered using a 0.45 μm membrane filter (MF) and placed onto Tryptone Bile X-Glucuronide (TBX) agar or CHROMagar ECC (ECC), or (2) tested by IDEXX Quanti-Tray/2000 Colilert. Recovery percentages of E. coli at both turbidity levels were similar on TBX and ECC, but pan-susceptible E. coli ARS C101 in TAW at 50 NTU on TBX was not quantifiable. For Colilert assays, the recovery percentages of E. coli from 0 NTU and 50 NTU TAW were between 93.4 ± 4.4% and 127.0 ± 10.5%. Overall, ECC and Colilert provided quantifiable results for recovery of pan-susceptible and cefotaxime-resistant E. coli from artificial agricultural water at different turbidity levels. Higher levels of turbidity affected the ability to quantify E. coli by MF with TBX and recovery by Colilert.

Indexed as

AgricultureAnti-Bacterial AgentsDrug Resistance, BacterialEscherichia coliFiltrationWater MicrobiologyMembranes, ArtificialAnti-Bacterial AgentsMembranes, ArtificialAgricultural waterAntimicrobial resistanceColilert assayEscherichia coliMembrane filtrationTest methods

Identifiers

PMID40835937
PMCPMC12366208

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.