ArticleMicrobes and environments2025
Improvements in the Duckweed-Microbe Co-cultivation Method for the Stable and Efficient Isolation of Rarely Cultivated Bacteria Using Microfilter Membranes.
Article in Microbes and environments, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- A cooperative cobalamide biosynthesis guild in the endosphere of the edible aquatic plant Wolffia globosa Mankai.Environmental microbiome · 2026Article
- Complete genome sequence of a member of a potentially novel genus within the family Opitutaceae isolated from a liquid medium used for co-cultivating duckweeds and river water-derived microbes.Microbiology resource announcements · 2025Article
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9 authors.
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Abstract
We recently proposed a novel microbial isolation technique, the "duckweed-microbe co-cultivation method", for isolating a wide variety of microbes, including rarely cultivated microbes. This method involves the inoculation of aseptic duckweed with environmental microbes followed by co-cultivation for a set period. Plants and their surrounding medium are then used as microbial sources for isolation in the conventional agar plate method. In the present study, we improved the method by using microfilter membranes (pore sizes of 0.8-1.2 μm) to pretreat microbial inocula, which increased the isolation efficiency of rarely cultivated microbes representing the phylum Verrucomicrobiota.
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