Evidence map›Paper›PMID 40834074›Full record

ArticleScience advances2025

Plant negative-strand RNA virus phosphoprotein condensates exploit host trafficking and lipid synthesis for viral factory assembly.

Zhiyi Wang, Jingyi Zhang, Jilei Huang, Gan Sha, Xinyue Song, Xue Cao, Zhenchen Yan, Chuanhe Liu, Siping Chen, Ziying Li and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science advances, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Zhiyi WangGuangdong Basic Research Center of Excellence for Precise Breeding of Future Crops, South China Agricultural University, Guangzhou 510642, China.ORCID 0009-0004-9891-0199
Jingyi ZhangGuangdong Basic Research Center of Excellence for Precise Breeding of Future Crops, South China Agricultural University, Guangzhou 510642, China.ORCID 0009-0003-3183-2432
Jilei HuangInstrumental Analysis and Research Center, South China Agricultural University, Guangzhou 510642, China.ORCID 0000-0002-5143-1981
Gan ShaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China.ORCID 0009-0008-1966-8656
Xinyue SongGuangdong Basic Research Center of Excellence for Precise Breeding of Future Crops, South China Agricultural University, Guangzhou 510642, China.
Xue CaoGuangdong Basic Research Center of Excellence for Precise Breeding of Future Crops, South China Agricultural University, Guangzhou 510642, China.
Zhenchen YanGuangdong Basic Research Center of Excellence for Precise Breeding of Future Crops, South China Agricultural University, Guangzhou 510642, China.
Chuanhe LiuInstrumental Analysis and Research Center, South China Agricultural University, Guangzhou 510642, China.
Siping ChenGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China.
Ziying LiGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China.
Xiuqin HuangGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China.
Qingjun XieGuangdong Basic Research Center of Excellence for Precise Breeding of Future Crops, South China Agricultural University, Guangzhou 510642, China.ORCID 0000-0002-6372-3260
Xin YangGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China.ORCID 0000-0001-9499-4170
Guohui ZhouGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China.ORCID 0000-0003-4266-414X
Tong ZhangGuangdong Basic Research Center of Excellence for Precise Breeding of Future Crops, South China Agricultural University, Guangzhou 510642, China.ORCID 0000-0002-5725-9510

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

RNA viruses often remodel host intracellular membranes to establish specialized replication compartments through viral protein-induced phase separation. However, the mechanisms underlying membrane remodeling and the characteristics that render these sites conducive to replication remain poorly understood, particularly in plant negative-strand RNA viruses. Here, we demonstrate that the phosphoprotein (P) of rice stripe mosaic virus (RSMV) forms biomolecular condensates via liquid-liquid phase separation (LLPS) to recruit essential components for viral replication factories (VFs). We identify a direct interaction between RSMV P and adenosine diphosphate (ADP) ribosylation factor 1 (OsARF1C), a crucial regulator of the coatomer protein I (COP I) vesicle transport pathway that is vital for viral replication. This interaction indirectly recruits OsARF1C's partner, phosphatidylinositol 4-kinase beta (OsPI4KB), which drives localized phosphatidylinositol-4 phosphate (PI4P) synthesis. Concurrently, the P protein modulates its aggregates and LLPS droplets through PI4P, thereby expanding the replication site and enhancing viral replication.

Indexed as

LipidsPhosphoproteinsPlant VirusesRNA VirusesViral ProteinsVirus AssemblyHost-Pathogen InteractionsOryzaPhosphatidylinositol PhosphatesPlant ProteinsVirus ReplicationLipidsphosphatidylinositol 4-phosphatePhosphatidylinositol PhosphatesPhosphoproteinsPlant ProteinsViral Proteins

Identifiers

PMID40834074
PMCPMC12366689

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.