Evidence map›Paper›PMID 40834073›Full record

ArticleScience advances2025

Tandem repeat-induced sexual silencing: A Rid-dependent RNAi mechanism for fungal genome defense via translational repression.

Mengde Hou, Yajia Ni, Jinrong Yin, Mengchun Wu, Helan Zhang, Yanfei Du, Shasha Chen, Zhipeng Zhou, Cong Jiang, Qinhu Wang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science advances, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Mengde HouState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.ORCID 0009-0004-9277-8238
Yajia NiState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
Jinrong YinState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
Mengchun WuState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
Helan ZhangState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
Yanfei DuState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.ORCID 0009-0008-7473-5129
Shasha ChenNational Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Zhipeng ZhouNational Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.ORCID 0000-0002-8449-7194
Cong JiangState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.ORCID 0000-0002-5634-3200
Qinhu WangState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.ORCID 0000-0003-1251-073X
Huiquan LiuState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.ORCID 0000-0002-4723-845X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Eukaryotes have evolved defense systems to protect their genomes from invasive genetic elements. We report a fungal defense mechanism, tandem repeat-induced sexual silencing (TRISS), active during sexual stages. TRISS is a unique RNA interference (RNAi) pathway distinct from known mechanisms like quelling and meiotic silencing by unpaired DNA (MSUD). Triggered by tandem repeats, it involves RNAi components similar to quelling but uniquely includes the MSUD-specific Argonaute Sms2. TRISS operates independently of recombination. We identified small interfering RNA sassociated with TRISS (trasiRNAs) in tandem repeats. Sms2, guided by trasiRNAs, mediates translational repression of target mRNAs and is crucial for trasiRNA biogenesis. TRISS requires the DNA methyltransferase Rid but not Rid-mediated repeat-induced point mutation (RIP). Both Sms2 and Rid interact with replication protein A (RPA) to recruit the RPA-Qde1/RdRP complex, dependent on the helicase Qde3. This study reveals a potentially conserved strategy linking RIP and RNAi to silence tandem repeats during fungal sexual stages, offering insights into fungal genome defense.

Indexed as

Genome, FungalProtein BiosynthesisRNA InterferenceTandem Repeat SequencesArgonaute ProteinsFungal ProteinsGene Expression Regulation, FungalRNA, Small InterferingArgonaute ProteinsFungal ProteinsRNA, Small Interfering

Identifiers

PMID40834073
PMCPMC12366691

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.