Evidence map›Paper›PMID 40833575›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

T4 DNA-Induced Reconstruction of Artificial Nuclei in Living Mouse Oocytes.

Nao Yonezawa, Yasushi Hiraoka, Tokuko Haraguchi, Kazuo Yamagata

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Nao YonezawaFaculty of Biology-Oriented Science and Technology, Kindai University, Kinokawa, Japan.
Yasushi HiraokaGraduate School of Frontier Biosciences, Osaka University, Suita, Japan.
Tokuko HaraguchiGraduate School of Frontier Biosciences, Osaka University, Suita, Japan.
Kazuo YamagataFaculty of Biology-Oriented Science and Technology, Kindai University, Kinokawa, Japan. yamagata@waka.kindai.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fertilization involves a specialized nuclear formation process distinct from that of somatic cells, and reconstitution methods are useful for understanding its underlying mechanisms. Recently, we successfully reconstructed a nuclear-like structure, termed an "artificial nucleus," in living mouse oocytes by microinjecting solutions of T4 DNA (~166 kbp). This achievement allowed us to identify the physicochemical properties necessary for nuclear formation. In this paper, we describe a method for constructing artificial nuclei using T4 DNA solutions, which will contribute to future studies on the mechanisms of nuclear formation.

Indexed as

Cell NucleusDNAOocytesAnimalsFemaleMiceMicroinjectionsDNAArtificial nucleusIndirect immunofluorescence stainingLive-cell imagingMicroinjectionMouse oocyteT4 DNA

Identifiers

PMID40833575

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.