Evidence map›Paper›PMID 40833574›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Use of Nucleoporin-Conjugated Beads to Study the Nuclear Pore Complex Assembly on the Nuclear Membrane.

Şükriye Bilir, Yasushi Hiraoka, Tokuko Haraguchi

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Şükriye BilirGraduate School of Frontier Biosciences, Osaka University, Suita, Japan.
Yasushi HiraokaGraduate School of Frontier Biosciences, Osaka University, Suita, Japan.
Tokuko HaraguchiGraduate School of Frontier Biosciences, Osaka University, Suita, Japan. haraguchi.tokuko.fbs@osaka-u.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reassembly of the nuclear pore complex (NPC) at the end of mitosis is a key event for establishing a functional nuclear envelope. However, the role of each NPC component (Nup) in NPC reassembly remained unclear. Here, we describe a method using artificial beads conjugated with an anti-GFP antibody. In this method, such beads are introduced into living cells expressing the Nup of interest fused with GFP to tether the specific Nup around the beads. By examining the Nup-conjugated beads in mitotic telophase cells, it is possible to investigate the role of the specific Nup of interest in NPC formation on the nuclear membrane.

Indexed as

MicrospheresNuclear EnvelopeNuclear PoreNuclear Pore Complex ProteinsGreen Fluorescent ProteinsHeLa CellsHumansMitosisGreen Fluorescent ProteinsNuclear Pore Complex ProteinsAnti-GFP antibody-conjugated beadLive-cell imagingLive CLEM imagingNuclear pore complexNucleoporinNup-bead

Identifiers

PMID40833574

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.