Evidence map›Paper›PMID 40833567›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Fast Super-Resolution Live Cell Imaging of Nuclear Membrane-Endoplasmic Reticulum Dynamics.

Swapnil Sahoo, Abdur Rahaman

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Swapnil SahooSchool of Biological Sciences, National Institute of Science Education and Research, Bhubaneswar, Odisha, India.ORCID https://orcid.org/0000-0002-2104-8615
Abdur RahamanSchool of Biological Sciences, National Institute of Science Education and Research, Bhubaneswar, Odisha, India. arahaman@niser.ac.in.ORCID https://orcid.org/0000-0002-8440-9633

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The nuclear membrane undergoes continuous remodelling during the life cycle of all eukaryotic cells. The endoplasmic reticulum remains continuous with the nuclear membrane and regulates this nuclear remodelling process. Here in this chapter, we describe in detail a protocol for high-speed imaging of nuclear membrane/endoplasmic reticulum dynamic structure in live cell using fluorescent endoplasmic reticulum resident marker (GFP-KDEL). We used super-resolution microscope to capture this dynamic event. This protocol will help visualize the dynamic changes in nuclear membrane/endoplasmic reticulum structure at different cell cycle stages and understand the role of various cellular factors in this process.

Indexed as

Endoplasmic ReticulumNuclear EnvelopeGreen Fluorescent ProteinsHeLa CellsHumansMicroscopy, FluorescenceGreen Fluorescent ProteinsEndoplasmic reticulumHigh-speed imagingLive cell imagingNuclear membraneSuper-resolution

Identifiers

PMID40833567

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.